Improved analysis of TCR[gamma][delta] variable region expression in humans

• Verfasst von: Christopoulos, Petros [VerfasserIn]
• Titel: Improved analysis of TCR[gamma][delta] variable region expression in humans
• Verf.angabe: Petros Christopoulos, Daniela Bukatz, Sylvia Kock, Miroslav Malkovsky, Jürgen Finke, Paul Fisch
• Umfang: 7 S.
• Fussnoten: Im Titel sind gamma und delta als griechische Buchstaben dargestellt ; Gesehen am 19.02.2018
• Titel Quelle: Enthalten in: Journal of immunological methods
• Jahr Quelle: 2016
• Band/Heft Quelle: 434(2016), S. 66-72
• ISSN Quelle: 1872-7905
• DOI: doi:10.1016/j.jim.2016.04.009
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1569981116

Abstract

Deeper understanding of γδ Τ cell increases in various clinical situations requires the assessment of TCRγ and δ variable (V) region gene expression and junctional diversity. Here we describe an improved TCRγ and δ spectratyping method used to study the γδ T-cell expansions in two patients with thymoma and immunodeficiency. One of these patients also suffered from chronic CMV infection and pure red cell aplasia and the other from chronic visceral leishmaniasis and myasthenia gravis. Analyses of the junctional diversity of the TCRγ and δ chains, flow cytometry with a panel of non-commercially available anti-TCRγδ V region monoclonal antibodies and functional studies were performed. The results clearly distinguished an expansion of oligoclonal, most likely antigen-driven, cytotoxic γδ T cells in the first patient from a naive pattern of polyclonal γδ T-cell proliferation in the second. These findings demonstrate the diversity of γδ T-cell expansions in immunodeficient patients and highlight the value of spectratyping as a tool for their characterization and understanding of the underlying mechanisms.