Status: Bibliographieeintrag
| Online-Ressource |
Verfasst von: | Kais, Britta [VerfasserIn]  |
| Ottermanns, Richard [VerfasserIn]  |
| Scheller, Franziska [VerfasserIn]  |
| Braunbeck, Thomas [VerfasserIn]  |
Titel: | Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A |
Verf.angabe: | Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck |
Umfang: | 18 S. |
Fussnoten: | Available online: 2 October 2017 ; Gesehen am 21.02.2018 |
Titel Quelle: | Enthalten in: The science of the total environment |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 615(2018), S. 330-347 |
ISSN Quelle: | 1879-1026 |
Abstract: | The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials. |
DOI: | doi:10.1016/j.scitotenv.2017.09.257 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Verlag: http://dx.doi.org/10.1016/j.scitotenv.2017.09.257 |
| Verlag: http://www.sciencedirect.com/science/article/pii/S0048969717326098 |
| DOI: https://doi.org/10.1016/j.scitotenv.2017.09.257 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Bibliogr. Hinweis: | Erscheint auch als Druck-Ausgabe: Kais, Britta: Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A |
K10plus-PPN: | 157007027X |
Verknüpfungen: | → Zeitschrift |
Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A / Kais, Britta [VerfasserIn] (Online-Ressource)
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