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Verfasst von:Kais, Britta [VerfasserIn]   i
 Ottermanns, Richard [VerfasserIn]   i
 Scheller, Franziska [VerfasserIn]   i
 Braunbeck, Thomas [VerfasserIn]   i
Titel:Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
Verf.angabe:Britta Kais, Richard Ottermanns, Franziska Scheller, Thomas Braunbeck
Umfang:18 S.
Fussnoten:Available online: 2 October 2017 ; Gesehen am 21.02.2018
Titel Quelle:Enthalten in: The science of the total environment
Jahr Quelle:2018
Band/Heft Quelle:615(2018), S. 330-347
ISSN Quelle:1879-1026
Abstract:The visualization of specific activation of the aryl hydrocarbon receptor (AhR) directly in the zebrafish embryo (Danio rerio) via live-imaging is a reliable tool to investigate the presence of dioxin-like substances in environmental samples. The co-existence of inducers and inhibitors of cytochrome P450-dependent monooxygenases (CYP1A) is typical of complex environmental mixtures and requires modifications of the in vivo EROD assay: For this end, zebrafish embryos were used to evaluate the EROD-modifying potentials of common single-compound exposures as well as binary mixtures with the PAH-type Ah-receptor agonist β-naphthoflavone. For chemical testing, chlorpyrifos and Aroclor 1254 were selected; β-naphthoflavone served as maximum EROD induction control. Chlorpyrifos (≤EC10) could be documented to be a strong CYP1A inhibitor causing characteristic edema-related toxicity. Aroclor 1254 resulted in inhibition of CYP1A catalytic activity in a concentration- and specific time-dependent manner. Next to a fast CYP1A induction, CYP1A inhibition could also be detected after 3h short-term exposure of zebrafish embryos to chlorpyrifos. This communication also describes techniques for the quantification of fluorescence signals via densitometry as a basis for subsequent statistical assessment. The co-exposure approach with zebrafish embryos accounts for the nature of potential interaction between CYP1A inducers and inhibitors and thus pays tribute to the complexity of environmental mixtures. The co-exposure EROD live-imaging assay thus facilitates a better understanding of mixture effects and allows a better assessment and interpretation of (embryo) toxic potentials.
DOI:doi:10.1016/j.scitotenv.2017.09.257
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1016/j.scitotenv.2017.09.257
 Verlag: http://www.sciencedirect.com/science/article/pii/S0048969717326098
 DOI: https://doi.org/10.1016/j.scitotenv.2017.09.257
Datenträger:Online-Ressource
Sprache:eng
Bibliogr. Hinweis:Erscheint auch als Druck-Ausgabe: Kais, Britta: Modification and quantification of in vivo EROD live-imaging with zebrafish (Danio rerio) embryos to detect both induction and inhibition of CYP1A
K10plus-PPN:157007027X
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