Low-dose nuclear microscopy as a necessity for accurate quantitative microanalysis of biological samples

• Verfasst von: Maetz, Mischa [VerfasserIn]
• Verfasst von: Schüßler, Arthur [VerfasserIn]
• Verfasst von: Traxel, Kurt [VerfasserIn]
• Titel: Low-dose nuclear microscopy as a necessity for accurate quantitative microanalysis of biological samples
• Verf.angabe: Mischa Maetz, Wojtek J. Przybylowicz, Jolanda Mesjasz-Przybylowicz, Arthur Schüßler, Kurt Traxel
• Umfang: 7 S.
• Fussnoten: Gesehen am 05.03.2018
• Titel Quelle: Enthalten in: Nuclear instruments & methods in physics research / B
• Jahr Quelle: 1999
• Band/Heft Quelle: 158(1999), 1, S. 292-298
• ISSN Quelle: 1872-9584
• DOI: doi:10.1016/S0168-583X(99)00331-6
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1570394423

Abstract

Some aspects of quantitative analysis of biological samples - including a review of the relevant literature - are discussed. Special emphasis is put on the accuracy of trace elemental results despite severe sample damage which occurs during analysis. The occuring mass loss can be corrected by combining quantitative STIM and PIXE. It is also possible to characterize the volatility of most of the minor and trace elements of the sample. The comparison between the presented model and matrix determination with RBS, a widely used method, definitely demonstrates that the concentrations obtained using RBS correction only may be too high by a factor of two. We recommend at least one low-dose analysis to do correct quantification.