Junb controls lymphatic vascular development in zebrafish via miR-182

• Verfasst von: Kiesow, Kristin [VerfasserIn]
• Verfasst von: Bennewitz, Katrin [VerfasserIn]
• Verfasst von: Cramer-Stoll, Sandra [VerfasserIn]
• Verfasst von: Kroll, Jens [VerfasserIn]
• Titel: Junb controls lymphatic vascular development in zebrafish via miR-182
• Verf.angabe: Kristin Kiesow, Katrin Bennewitz, Laura Gutierrez Miranda, Sandra J. Stoll, Bettina Hartenstein, Peter Angel, Jens Kroll, Marina Schorpp-Kistner
• E-Jahr: 2015
• Jahr: 13 October 2015
• Umfang: 16 S.
• Fussnoten: Gesehen am 15.03.2018
• Titel Quelle: Enthalten in: Scientific reports
• Ort Quelle: [London] : Springer Nature, 2011
• Jahr Quelle: 2015
• Band/Heft Quelle: 5(2015) Artikel-Nummer 15007, 16 Seiten
• ISSN Quelle: 2045-2322
• DOI: doi:10.1038/srep15007
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1571101187

Abstract

JUNB, a subunit of the AP-1 transcription factor complex, mediates gene regulation in response to a plethora of extracellular stimuli. Previously, JUNB was shown to act as a critical positive regulator of blood vessel development and homeostasis as well as a negative regulator of proliferation, inflammation and tumour growth. Here, we demonstrate that the oncogenic miR-182 is a novel JUNB target. Loss-of-function studies by morpholino-mediated knockdown and the CRISPR/Cas9 technology identify a novel function for both JUNB and its target miR-182 in lymphatic vascular development in zebrafish. Furthermore, we show that miR-182 attenuates foxo1 expression indicating that strictly balanced Foxo1 levels are required for proper lymphatic vascular development in zebrafish. In conclusion, our findings uncover with the Junb/miR-182/Foxo1 regulatory axis a novel key player in governing lymphatic vascular morphogenesis in zebrafish.