P16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya

• Verfasst von: Wangari Ngugi, Caroline [VerfasserIn]
• Verfasst von: Schmidt, Dietmar [VerfasserIn]
• Verfasst von: Knebel Doeberitz, Magnus von [VerfasserIn]
• Verfasst von: Reuschenbach, Miriam [VerfasserIn]
• Titel: P16INK4a/Ki-67 dual stain cytology for cervical cancer screening in Thika district, Kenya
• Verf.angabe: Caroline Wangari Ngugi, Dietmar Schmidt, Karanja Wanyoro, Hamadi Boga, Peter Wanzala, Anne Muigai, John Mbithi, Magnus von Knebel Doeberitz and Miriam Reuschenbach
• E-Jahr: 2015
• Jahr: 11 August 2015
• Umfang: 6$p10(2015) Artikel-Nummer 25, 6 Seiten
• Fussnoten: Gesehen am 15.03.2018 ; Im Titel ist "INK4a" hochgestellt
• Titel Quelle: Enthalten in: Infectious agents and cancer
• Ort Quelle: London : Biomed Central, 2006
• Jahr Quelle: 2015
• Band/Heft Quelle: 10(2015)
• ISSN Quelle: 1750-9378
• DOI: doi:10.1186/s13027-015-0020-2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Cervical cancer
• Sach-SW: Developing countries
• Sach-SW: HPV
• Sach-SW: Ki-67
• Sach-SW: p16INK4a
• Sach-SW: Screening
• K10plus-PPN: 1571108351

Abstract

Background: The identification of suited early detection tests is one among the multiple requirements to reduce cervical cancer incidence in developing countries. Methods: We evaluated p16INK4a/Ki-67 dual-stain cytology in a screening population in Thika district, Kenya and compared it to high-risk human papillomavirus (HR-HPV) DNA testing and visual inspection by acetic acid (VIA) and Lugol’s iodine (VILI). Results: Valid results for all tests could be obtained in 477 women. 20.9 % (100/477) were tested positive for HR-HPV DNA, 3.1 % (15/477) had positive VIA/VILI and 8.2 % (39/477) positive p16INK4a/Ki-67 cytology. Of 22 women that showed up for colposcopy and biopsy, 6 women were diagnosed with CIN3 and two with CIN2. All women with CIN2/3 were negative in VIA/VILI screening and positive by HR-HPV DNA testing. But HPV was also positive in 91.7 % (11/12) of women with normal histology. p16INK4a/Ki-67 cytology was positive in all 6 women with CIN3, in one of the two CIN2 and in only 8.3 % (1/12) of women with normal histology. Conclusions: p16INK4a/Ki-67 cytology is an interesting test for further studies in developing countries, since our findings point to a lower fraction of false positive test results using p16INK4a/Ki-67 cytology compared to HPV DNA testing in a Kenyan screening population. VIA/VILI missed all histology-proven CIN2/3.