Central metabolite changes and activation of microglia after peripheral interleukin-2 challenge

• Verfasst von: Schneider, Peggy [VerfasserIn]
• Verfasst von: Sartorius, Alexander [VerfasserIn]
• Verfasst von: Spanagel, Rainer [VerfasserIn]
• Titel: Central metabolite changes and activation of microglia after peripheral interleukin-2 challenge
• Verf.angabe: Peggy Schneider, Wolfgang Weber-Fahr, Nina Schweinfurth, Ying-Jui Ho, Alexander Sartorius, Rainer Spanagel, Cornelius R. Pawlak
• E-Jahr: 2012
• Jahr: February 2012
• Umfang: 7 S.
• Fussnoten: Gesehen am 06.04.2018
• Titel Quelle: Enthalten in: Brain, behavior and immunity
• Ort Quelle: Orlando, Fla. [u.a.] : Elsevier, 1987
• Jahr Quelle: 2012
• Band/Heft Quelle: 26(2012), 2, Seite 277-283
• ISSN Quelle: 1090-2139
• DOI: doi:10.1016/j.bbi.2011.09.011
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Anxiety
• Sach-SW: -inositol
• Sach-SW: CD25
• Sach-SW: FACS
• Sach-SW: H MR spectroscopy
• Sach-SW: Interleukin (IL)-2
• Sach-SW: Microglia
• Sach-SW: Rats
• K10plus-PPN: 1571769641

Abstract

Interleukin (IL)-2 regulates the immune response through the proliferation of activated T-cells and also exerts effects on the central nervous system (CNS). Alongside having marked neurobehavioral effects, IL-2 has been suggested to impact on various psychiatric disorders. The immune-CNS communication of IL-2 remains unclear, although, it is suggested that microglia are the source and target of IL-2. Here, we analyzed changes in brain metabolites following a peripheral IL-2 challenge and examined the contribution of microglia in mediating these effects. Rats were assessed by magnetic resonance spectroscopy (MRS) in a 9.4T scanner for baseline metabolite levels in the prefrontal cortex (PFC) and the hippocampus. After 7days animals were scanned again following a single injection of IL-2 (2.5μg/kg) and then tested on the elevated plus-maze for the correlation of IL-2-induced brain metabolites and measures of anxiety. In another experiment CD25+ microglia cells were determined. A separate group of rats was injected either with IL-2 or vehicle, and afterward the PFC and hippocampus were dissected and fluorescence activated cell sorting (FACS) analysis was performed. The MRS scans in the intra-individual study design showed a significant increase in myo-inositol in the analyzed regions. A significant correlation of anxiety-like measures and myo-inositol, a marker for microglia activity, was found in the hippocampus. The FACS analysis showed a significant increase in CD25+ microglia in the hippocampus compared to controls. The results support the role of microglia as a mediator in the immune-CNS communication and the effects of peripheral IL-2.