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| Verfasst von: | Calviño, Fabiola R. [VerfasserIn]  |
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| | Kornprobst, Markus [VerfasserIn] |
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| | Schermann, Géza [VerfasserIn] |
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| | Birkle, Fabienne [VerfasserIn] |
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| | Wild, Klemens [VerfasserIn] |
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| | Fischer, Tamás [VerfasserIn] |
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| | Hurt, Ed [VerfasserIn] |
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| | Ahmed, Yasar Luqman [VerfasserIn] |
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| | Sinning, Irmgard [VerfasserIn] |
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| Titel: | Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis |
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| Verf.angabe: | Fabiola R. Calviño, Markus Kornprobst, Géza Schermann, Fabienne Birkle, Klemens Wild, Tamas Fischer, Ed Hurt, Yasar Luqman Ahmed, Irmgard Sinning |
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| E-Jahr: | 2017 |
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| Jahr: | June 2, 2017 |
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| Umfang: | 16 S. |
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| Fussnoten: | Gesehen am 11.04.2018 |
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| Titel Quelle: | Enthalten in: PLOS ONE |
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| Ort Quelle: | San Francisco, California, US : PLOS, 2006 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 12(2017,6) Artikel-Nummer e0178752, 16 Seiten |
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| ISSN Quelle: | 1932-6203 |
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| Abstract: | Eukaryotic ribosome biogenesis begins with the co-transcriptional assembly of the 90S pre-ribosome. The ‘U three protein’ (UTP) complexes and snoRNP particles arrange around the nascent pre-ribosomal RNA chaperoning its folding and further maturation. The earliest event in this hierarchical process is the binding of the UTP-A complex to the 5'-end of the pre-ribosomal RNA (5'-ETS). This oligomeric complex predominantly consists of β-propeller and α-solenoidal proteins. Here we present the structure of the Utp4 subunit from the thermophilic fungus Chaetomium thermophilum at 2.15 Å resolution and analyze its function by UV RNA-crosslinking (CRAC) and in context of a recent cryo-EM structure of the 90S pre-ribosome. Utp4 consists of two orthogonal and highly basic β-propellers that perfectly fit the EM-data. The Utp4 structure highlights an unusual Velcro-closure of its C-terminal β-propeller as relevant for protein integrity and potentially Utp8 recognition in the context of the pre-ribosome. We provide a first model of the 5'-ETS RNA from the internally hidden 5'-end up to the region that hybridizes to the 3'-hinge sequence of U3 snoRNA and validate a specific Utp4/5'-ETS interaction by CRAC analysis. |
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| DOI: | doi:10.1371/journal.pone.0178752 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1371/journal.pone.0178752 |
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| | Kostenfrei: Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0178752 |
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| | DOI: https://doi.org/10.1371/journal.pone.0178752 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Biosynthesis |
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| | Crystal structure |
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| | Electron cryo-microscopy |
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| | Fungal structure |
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| | Ribosomal RNA |
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| | Ribosomes |
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| | Saccharomyces cerevisiae |
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| | Small nucleolar RNAs |
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| K10plus-PPN: | 1571892885 |
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| Verknüpfungen: | → Zeitschrift |
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Structural basis for 5'-ETS recognition by Utp4 at the early stages of ribosome biogenesis / Calviño, Fabiola R. [VerfasserIn]; June 2, 2017 (Online-Ressource)
