ABC transporter function and regulation at the blood-spinal cord barrier

• Verfasst von: Campos, Christopher R [VerfasserIn]
• Verfasst von: Schröter, Christian [VerfasserIn]
• Titel: ABC transporter function and regulation at the blood-spinal cord barrier
• Verf.angabe: Christopher R Campos, Christian Schröter, Xueqian Wang, David S Miller
• Umfang: 8 S.
• Fussnoten: Gesehen am 13.04.2018
• Titel Quelle: Enthalten in: Journal of cerebral blood flow & metabolism
• Jahr Quelle: 2012
• Band/Heft Quelle: 32(2012), 8, S. 1559-1566
• ISSN Quelle: 1559-7016
• DOI: doi:10.1038/jcbfm.2012.47
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1571984852

Abstract

We present here an initial characterization of ATP binding cassette (ABC) transporter function and regulation at the blood-spinal cord barrier. We isolated capillaries from rat spinal cords and studied transport function using a confocal microscopy-based assay and protein expression using western blots. These capillaries exhibited transport function and protein expression of P-glycoprotein (Abcb1), multidrug resistance protein 2 (Mrp2, Abcc2), and breast cancer-related protein (Bcrp, Abcg2). Exposing isolated capillaries to dioxin (activates aryl hydrocarbon receptor) increased transport mediated by all three transporters. Brain and spinal cord capillaries from dioxin-dosed rats exhibited increased P-glycoprotein-mediated transport and increased protein expression for all three ABC transporters. These findings indicate similar ABC transporter expression, function, and regulation at the blood-spinal cord and blood-brain barriers.