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Verfasst von:Dinner, Stefanie [VerfasserIn]   i
 Kaltschmidt, Julian [VerfasserIn]   i
 Stump-Guthier, Carolin [VerfasserIn]   i
 Hetjens, Svetlana [VerfasserIn]   i
 Tenenbaum, Tobias [VerfasserIn]   i
 Schroten, Horst [VerfasserIn]   i
 Schwerk, Christian [VerfasserIn]   i
Titel:Mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by Listeria monocytogenes
Verf.angabe:Stefanie Dinner, Julian Kaltschmidt, Carolin Stump-Guthier, Svetlana Hetjens, Hiroshi Ishikawa, Tobias Tenenbaum, Horst Schroten, Christian Schwerk
Jahr:2017
Umfang:16 S.
Fussnoten:Published online: 23 September 2016 ; Gesehen am 18.04.2018
Titel Quelle:Enthalten in: Microbes and infection
Ort Quelle:Paris [u.a.] : Elsevier, 1999
Jahr Quelle:2017
Band/Heft Quelle:19(2017), 1, Seite 18-33
ISSN Quelle:1769-714X
Abstract:Listeria monocytogenes, a Gram-positive bacterium, can cause meningitis after invading the human central nervous system. The blood-cerebrospinal fluid barrier (BCSFB), located at the epithelium of the choroid plexus, is a possible entry site for L. monocytogenes into the brain, and in vitro L. monocytogenes invades human choroid plexus epithelial papilloma (HIBCPP) cells. Although host cell signal transduction subsequent to infection by L. monocytogenes has been investigated, the role of mitogen-activated protein kinases (MAPK) is not clarified yet. We show that infection with L. monocytogenes causes activation of the MAPKs Erk1/2 and p38 preferentially when bacteria are added to the physiologically more relevant basolateral side of HIBCPP cells. Deletion of the listerial virulence factors Internalin (InlA) and InlB reduces MAPK activation. Whereas inhibition of either Erk1/2 or p38 signaling significantly attenuates infection of HIBCPP cells with L. monocytogenes, simultaneous inhibition of both MAPK pathways shows an additive effect, and Erk1/2 and p38 are involved in regulation of cytokine and chemokine expression following infection. Blocking of endocytosis with the synthetic dynamin inhibitor dynasore strongly abrogates infection of HIBCPP cells with L. monocytogenes. Concurrent inhibition of MAPK signaling further reduces infection, suggesting MAPKs mediate infection with L. monocytogenes during inhibition of dynamin-mediated endocytosis.
DOI:doi:10.1016/j.micinf.2016.09.003
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.micinf.2016.09.003
 Volltext: http://www.sciencedirect.com/science/article/pii/S1286457916301307
 DOI: https://doi.org/10.1016/j.micinf.2016.09.003
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Blood-cerebrospinal fluid barrier
 Choroid plexus
 Dynamin
 Human
 Mitogen-activated protein kinase
K10plus-PPN:1572132434
Verknüpfungen:→ Zeitschrift

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