Mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by Listeria monocytogenes

• Verfasst von: Dinner, Stefanie [VerfasserIn]
• Verfasst von: Kaltschmidt, Julian [VerfasserIn]
• Verfasst von: Stump-Guthier, Carolin [VerfasserIn]
• Verfasst von: Hetjens, Svetlana [VerfasserIn]
• Verfasst von: Tenenbaum, Tobias [VerfasserIn]
• Verfasst von: Schroten, Horst [VerfasserIn]
• Verfasst von: Schwerk, Christian [VerfasserIn]
• Titel: Mitogen-activated protein kinases are required for effective infection of human choroid plexus epithelial cells by Listeria monocytogenes
• Verf.angabe: Stefanie Dinner, Julian Kaltschmidt, Carolin Stump-Guthier, Svetlana Hetjens, Hiroshi Ishikawa, Tobias Tenenbaum, Horst Schroten, Christian Schwerk
• Jahr: 2017
• Umfang: 16 S.
• Fussnoten: Published online: 23 September 2016 ; Gesehen am 18.04.2018
• Titel Quelle: Enthalten in: Microbes and infection
• Ort Quelle: Paris [u.a.] : Elsevier, 1999
• Jahr Quelle: 2017
• Band/Heft Quelle: 19(2017), 1, Seite 18-33
• ISSN Quelle: 1769-714X
• DOI: doi:10.1016/j.micinf.2016.09.003
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Blood-cerebrospinal fluid barrier
• Sach-SW: Choroid plexus
• Sach-SW: Dynamin
• Sach-SW: Human
• Sach-SW: Mitogen-activated protein kinase
• K10plus-PPN: 1572132434

Abstract

Listeria monocytogenes, a Gram-positive bacterium, can cause meningitis after invading the human central nervous system. The blood-cerebrospinal fluid barrier (BCSFB), located at the epithelium of the choroid plexus, is a possible entry site for L. monocytogenes into the brain, and in vitro L. monocytogenes invades human choroid plexus epithelial papilloma (HIBCPP) cells. Although host cell signal transduction subsequent to infection by L. monocytogenes has been investigated, the role of mitogen-activated protein kinases (MAPK) is not clarified yet. We show that infection with L. monocytogenes causes activation of the MAPKs Erk1/2 and p38 preferentially when bacteria are added to the physiologically more relevant basolateral side of HIBCPP cells. Deletion of the listerial virulence factors Internalin (InlA) and InlB reduces MAPK activation. Whereas inhibition of either Erk1/2 or p38 signaling significantly attenuates infection of HIBCPP cells with L. monocytogenes, simultaneous inhibition of both MAPK pathways shows an additive effect, and Erk1/2 and p38 are involved in regulation of cytokine and chemokine expression following infection. Blocking of endocytosis with the synthetic dynamin inhibitor dynasore strongly abrogates infection of HIBCPP cells with L. monocytogenes. Concurrent inhibition of MAPK signaling further reduces infection, suggesting MAPKs mediate infection with L. monocytogenes during inhibition of dynamin-mediated endocytosis.