Crossreactivity of an antiserum directed to the gram-negative bacterium Neisseria gonorrhoeae with the SNARE-complex protein Snap23 correlates to impaired exocytosis in SH-SY5Y cells

• Verfasst von: Almamy, Abdullah [VerfasserIn]
• Verfasst von: Schwerk, Christian [VerfasserIn]
• Verfasst von: Schroten, Horst [VerfasserIn]
• Titel: Crossreactivity of an antiserum directed to the gram-negative bacterium Neisseria gonorrhoeae with the SNARE-complex protein Snap23 correlates to impaired exocytosis in SH-SY5Y cells
• Verf.angabe: A. Almamy, C. Schwerk, H. Schroten, H. Ishikawa, A. R. Asif, B. Reuss
• E-Jahr: 2017
• Jahr: 01 May 2017
• Umfang: 18 S.
• Fussnoten: Gesehen am 30.04.2018
• Titel Quelle: Enthalten in: Journal of molecular neuroscience
• Ort Quelle: New York, NY : Springer, 1998
• Jahr Quelle: 2017
• Band/Heft Quelle: 62(2017), 2, Seite 163-180
• ISSN Quelle: 1559-1166
• DOI: doi:10.1007/s12031-017-0920-2
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Antibodies, Bacterial
• Sach-SW: Brain
• Sach-SW: Cell Line, Tumor
• Sach-SW: Exocytosis
• Sach-SW: Glucose Transporter Type 4
• Sach-SW: GluT4
• Sach-SW: Humans
• Sach-SW: Immune Sera
• Sach-SW: Neisseria gonorrhoeae
• Sach-SW: Neisseria meningitidis
• Sach-SW: Neurons
• Sach-SW: Qb-SNARE Proteins
• Sach-SW: Qc-SNARE Proteins
• Sach-SW: SH-SY5Y cells
• Sach-SW: Snap23
• K10plus-PPN: 1572482737

Abstract

Early maternal infections with Neisseria gonorrhoeae (NG) correlate to an increased lifetime schizophrenia risk for the offspring, which might be due to an immune-mediated mechanism. Here, we investigated the interactions of polyclonal antisera to NG (α-NG) with a first trimester prenatal brain multiprotein array, revealing among others the SNARE-complex protein Snap23 as a target antigen for α-NG. This interaction was confirmed by Western blot analysis with a recombinant Snap23 protein, whereas the closely related Snap25 failed to interact with α-NG. Furthermore, a polyclonal antiserum to the closely related bacterium Neisseria meningitidis (α-NM) failed to interact with both proteins. Functionally, in SH-SY5Y cells, α-NG pretreatment interfered with both insulin-induced vesicle recycling, as revealed by uptake of the fluorescent endocytosis marker FM1-43, and insulin-dependent membrane translocation of the glucose transporter GluT4. Similar effects could be observed for an antiserum raised directly to Snap23, whereas a serum to Snap25 failed to do so. In conclusion, Snap23 seems to be a possible immune target for anti-gonococcal antibodies, the interactions of which seem at least in vitro to interfere with vesicle-associated exocytosis. Whether these changes contribute to the correlation between maternal gonococcal infections and psychosis in vivo remains still to be clarified.