A tupaia paramyxovirus vector system for targeting and transgene expression

• Verfasst von: Engeland, Christine Elisabeth [VerfasserIn]
• Verfasst von: Bossow, Sascha [VerfasserIn]
• Verfasst von: Hoyler, Birgit [VerfasserIn]
• Verfasst von: Förster, Judith [VerfasserIn]
• Verfasst von: Veinalde, Rūta [VerfasserIn]
• Verfasst von: Jäger, Dirk [VerfasserIn]
• Verfasst von: Ungerechts, Guy [VerfasserIn]
• Verfasst von: Springfeld, Christoph [VerfasserIn]
• Titel: A tupaia paramyxovirus vector system for targeting and transgene expression
• Verf.angabe: Christine E. Engeland, Sascha Bossow, Andrew W. Hudacek, Birgit Hoyler, Judith Förster, Rūta Veinalde, Dirk Jäger, Roberto Cattaneo, Guy Ungerechts, Christoph Springfeld
• E-Jahr: 2017
• Jahr: 08 September 2017
• Umfang: 10 S.
• Fussnoten: Gesehen am 09.05.2018
• Titel Quelle: Enthalten in: Journal of general virology
• Ort Quelle: Reading : Soc., 1967
• Jahr Quelle: 2017
• Band/Heft Quelle: 98(2017), 9, Seite 2248-2257
• ISSN Quelle: 1465-2099
• DOI: doi:10.1099/jgv.0.000887
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1574285068

Abstract

Viruses from the diverse family of Paramyxoviridae include important pathogens and are applied in gene therapy and for cancer treatment. The Tupaia paramyxovirus (TPMV), isolated from the kidney of a tree shrew, does not infect human cells and neutralizing antibodies against other Paramyxoviridae do not cross-react with TPMV. Here, we present a vector system for de novo generation of infectious TPMV that allows for insertion of additional genes as well as targeting using antibody single-chain variable fragments. We show that the recombinant TPMV specifically infect cells expressing the targeted receptor and replicate in human cells. This vector system provides a valuable tool for both basic research and therapeutic applications.