Cryo-Electron Microscopy Modeling by the Molecular Dynamics Flexible Fitting Method

• Verfasst von: Chan, Kwok-Yan [VerfasserIn]
• Verfasst von: Trabuco, Leonardo G. [VerfasserIn]
• Verfasst von: Schreiner, Eduard [VerfasserIn]
• Verfasst von: Schulten, Klaus [VerfasserIn]
• Titel: Cryo-Electron Microscopy Modeling by the Molecular Dynamics Flexible Fitting Method
• Verf.angabe: Kwok-Yan Chan, Leonardo G. Trabuco, Eduard Schreiner, Klaus Schulten
• Umfang: 25 S.
• Fussnoten: Gesehen am 15.05.2018
• Titel Quelle: Enthalten in: Biopolymers
• Jahr Quelle: 2012
• Band/Heft Quelle: 97(2012), 9, S. 678-686
• ISSN Quelle: 1097-0282
• DOI: doi:10.1002/bip.22042
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1575066726

Abstract

The increasing power and popularity of cryo-electron microscopy (cryo-EM) in structural biology brought about the development of so-called hybrid methods, which permit the interpretation of cryo-EM density maps beyond their nominal resolution in terms of atomic models. The Cryo-EM Modeling Challenge 2010 is the first community effort to bring together developers of hybrid methods as well as cryo-EM experimentalists. Participating in the challenge, the molecular dynamics flexible fitting (MDFF) method was applied to a number of cryo-EM density maps. The results are described here with special emphasis on the use of symmetry-based restraints to improve the quality of atomic models derived from density maps of symmetric complexes; on a comparison of the stereochemical quality of atomic models resulting from different hybrid methods; and on application of MDFF to electron crystallography data. © 2012 Wiley Periodicals, Inc. Biopolymers 97: 678-686, 2012.