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Verfasst von:Bowen, Thomas Scott [VerfasserIn]   i
 Gasch, Alexander [VerfasserIn]   i
 Labeit, Siegfried [VerfasserIn]   i
Titel:Small-molecule inhibition of MuRF1 attenuates skeletal muscle atrophy and dysfunction in cardiac cachexia
Verf.angabe:Thomas Scott Bowen, Volker Adams, Sarah Werner, Tina Fischer, Paulien Vinke, Maria Noel Brogger, Norman Mangner, Axel Linke, Peter Sehr, Joe Lewis, Dittmar Labeit, Alexander Gasch, and Siegfried Labeit
E-Jahr:2017
Jahr:8 September 2017
Umfang:15 S.
Fussnoten:Gesehen am 16.05.2018
Titel Quelle:Enthalten in: Journal of cachexia, sarcopenia and muscle
Ort Quelle:Hoboken, NJ : Wiley, 2010
Jahr Quelle:2017
Band/Heft Quelle:8(2017), 6, Seite 939-953
ISSN Quelle:2190-6009
Abstract:Background Muscle ring finger 1 (MuRF1) is a muscle-specific ubiquitin E3 ligase activated during clinical conditions associated with skeletal muscle wasting. Yet, there remains a paucity of therapeutic interventions that directly inhibit MuRF1 function, particularly in vivo. The current study, therefore, developed a novel compound targeting the central coiled coil domain of MuRF1 to inhibit muscle wasting in cardiac cachexia. Methods We identified small molecules that interfere with the MuRF1-titin interaction from a 130 000 compound screen based on Alpha Technology. A subset of nine prioritized compounds were synthesized and administrated during conditions of muscle wasting, that is, to C2C12 muscle cells treated with dexamethasone and to mice treated with monocrotaline to induce cardiac cachexia. Results The nine selected compounds inhibited MuRF1-titin complexation with IC50 values <25 μM, of which three were found to also inhibit MuRF1 E3 ligase activity, with one further showing low toxicity on cultured myotubes. This last compound, EMBL chemical core ID#704946, also prevented atrophy in myotubes induced by dexamethasone and attenuated fibre atrophy and contractile dysfunction in mice during cardiac cachexia. Proteomic and western blot analyses showed that stress pathways were attenuated by ID#704946 treatment, including down-regulation of MuRF1 and normalization of proteins associated with apoptosis (BAX) and protein synthesis (elF2B-delta). Furthermore, actin ubiquitinylation and proteasome activity was attenuated. Conclusions We identified a novel compound directed to MuRF1's central myofibrillar protein recognition domain. This compound attenuated in vivo muscle wasting and contractile dysfunction in cardiac cachexia by protecting de novo protein synthesis and by down-regulating apoptosis and ubiquitin-proteasome-dependent proteolysis.
DOI:doi:10.1002/jcsm.12233
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: http://dx.doi.org/10.1002/jcsm.12233
 kostenfrei: Volltext: https://onlinelibrary.wiley.com/doi/abs/10.1002/jcsm.12233
 DOI: https://doi.org/10.1002/jcsm.12233
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Chemical biology
 Diaphragm
 Muscle wasting
 Myofibrillar proteins
 Protein synthesis
 Ubiquitin-proteasome system
K10plus-PPN:1575170922
Verknüpfungen:→ Zeitschrift

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