| Online-Ressource |
Verfasst von: | Kats, Ilia [VerfasserIn]  |
| Khmelinskii, Anton [VerfasserIn]  |
| Kschonsak, Marc [VerfasserIn]  |
| Huber, Florian [VerfasserIn]  |
| Knieß, Robert A. [VerfasserIn]  |
| Bartosik, Anna [VerfasserIn]  |
| Knop, Michael [VerfasserIn]  |
Titel: | Mapping degradation signals and pathways in a eukaryotic N-terminome |
Verf.angabe: | authors Ilia Kats, Anton Khmelinskii, Marc Kschonsak, Florian Huber, Robert A. Knieß, Anna Bartosik, Michael Knop |
E-Jahr: | 2018 |
Jahr: | May 3, 2018 |
Umfang: | 14 S. |
Fussnoten: | Gesehen am 24.05.2017 |
Titel Quelle: | Enthalten in: Molecular cell |
Ort Quelle: | [Cambridge, Mass.] : Cell Press, 1997 |
Jahr Quelle: | 2018 |
Band/Heft Quelle: | 70(2018), 3, Seite 488-501.e5 |
ISSN Quelle: | 1097-4164 |
Abstract: | Summary: Most eukaryotic proteins are N-terminally acetylated. This modification can be recognized as a signal for selective protein degradation (degron) by the N-end rule pathways. However, the prevalence and specificity of such degrons in the proteome are unclear. Here, by systematically examining how protein turnover is affected by N-terminal sequences, we perform a comprehensive survey of degrons in the yeast N-terminome. We find that approximately 26% of nascent protein N termini encode cryptic degrons. These degrons exhibit high hydrophobicity and are frequently recognized by the E3 ubiquitin ligase Doa10, suggesting a role in protein quality control. In contrast, N-terminal acetylation rarely functions as a degron. Surprisingly, we identify two pathways where N-terminal acetylation has the opposite function and blocks protein degradation through the E3 ubiquitin ligase Ubr1. Our analysis highlights the complexity of N-terminal degrons and argues that hydrophobicity, not N-terminal acetylation, is the predominant feature of N-terminal degrons in nascent proteins. |
DOI: | doi:10.1016/j.molcel.2018.03.033 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.molcel.2018.03.033 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S1097276518302363 |
| DOI: https://doi.org/10.1016/j.molcel.2018.03.033 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Bibliogr. Hinweis: | Forschungsdaten: Kats, Ilia: Mapping degradation signals and pathways in a eukaryotic N-terminome [data set] |
Sach-SW: | deep sequencing |
| massively parallel protein turnover assays |
| multiplexed protein stability profiling |
| N-end rule |
| N-terminal acetylation |
| N-terminal methionine excision |
| N-terminal processing |
| protein quality control |
| selective protein degradation |
K10plus-PPN: | 1575491036 |
Verknüpfungen: | → Zeitschrift |
Mapping degradation signals and pathways in a eukaryotic N-terminome / Kats, Ilia [VerfasserIn]; May 3, 2018 (Online-Ressource)