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Verfasst von:Saar, Matthias [VerfasserIn]   i
 Grobholz, Rainer [VerfasserIn]   i
Titel:Experimental orthotopic prostate tumor in nude mice
Titelzusatz:techniques for local cell inoculation and three-dimensional ultrasound monitoring
Verf.angabe:Matthias Saar, M.D., Christina Körbel, D.V.M., Volker Jung, Ph.D., Henrik Suttmann, M.D., Rainer Grobholz, M.D., Michael Stöckle, M.D., Gerhard Unteregger, Ph.D., Michael D. Menger, M.D., Jörn Kamradt, M.D.
Jahr:2012
Umfang:9 S.
Fussnoten:Gesehen am 30.05.2018
Titel Quelle:Enthalten in: Urologic oncology
Ort Quelle:Amsterdam [u.a.] : Elsevier Science, 1995
Jahr Quelle:2012
Band/Heft Quelle:30(2012), 3, Seite 330-338
ISSN Quelle:1873-2496
Abstract:Objectives: Orthotopic prostate cancer models are of great importance for cancer research. Orthotopic models in mice have been described previously. However, these studies lack a detailed methodological description and fail to define standards for local cell inoculation. Herein, we studied the effect of different protocols on tumor growth and report for the first time the use of high resolution ultrasound for monitoring of tumor growth. Materials and methods: Orthotopic inoculation of DU 145 MN1 prostate cancer cells was performed in 30 nude mice varying (1) the amount of cells (5 × 105 vs. 5 × 104), (2) the number of puncture sites, and (3) the addition of matrigel. Surgical complications such as recoil of cells through the injection canal and rupture of the prostatic capsule were monitored. Animals were tracked by ultrasound imaging after 4, 5, and 6 weeks. Autopsy and histology confirmed local tumor growth. Results: A take rate of 27/30 (90%) was observed. Growth of orthotopic prostate tumors was increased after inoculation of a large amount of cells under the capsule of 1 dorsal prostate lobe, but inoculation of small amounts of cells still induced local tumors. Noninvasive ultrasound examination allowed to identify orthotopic tumor formation and to monitor tumor growth in vivo. Addition of matrigel did not accelerate tumor growth. Complications like recoil (6.8%) or rupture of the prostate capsule (1.4%) were rare. Conclusions: Inoculation of DU 145 MN1 cells under the prostate capsule with a defined procedure results in very high take rates. Ultrasound screening is feasible to repetitively monitor tumor growth.
DOI:doi:10.1016/j.urolonc.2010.02.014
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext ; Verlag: http://dx.doi.org/10.1016/j.urolonc.2010.02.014
 Volltext: http://www.sciencedirect.com/science/article/pii/S1078143910000566
 DOI: https://doi.org/10.1016/j.urolonc.2010.02.014
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Orthotopic tumor growth
 Prostate cancer
 Ultrasound
 Xenograft model
K10plus-PPN:1575835452
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