Sensitive and non-invasive assessment of hepatocellular iron using a novel room-temperature susceptometer

• Verfasst von: Müller, Johannes [VerfasserIn]
• Verfasst von: Raisi, Hanna [VerfasserIn]
• Verfasst von: Rausch, Vanessa [VerfasserIn]
• Verfasst von: Peccerella, Teresa [VerfasserIn]
• Verfasst von: Simons, David [VerfasserIn]
• Verfasst von: Ziener, Christian H. [VerfasserIn]
• Verfasst von: Schlemmer, Heinz-Peter [VerfasserIn]
• Verfasst von: Seitz, Helmut K. [VerfasserIn]
• Verfasst von: Waldburger, Nina [VerfasserIn]
• Verfasst von: Longerich, Thomas [VerfasserIn]
• Verfasst von: Straub, Beate Katharina [VerfasserIn]
• Verfasst von: Mueller, Sebastian [VerfasserIn]
• Titel: Sensitive and non-invasive assessment of hepatocellular iron using a novel room-temperature susceptometer
• Verf.angabe: Johannes Mueller, Hanna Raisi, Vanessa Rausch, Teresa Peccerella, David Simons, Christian Herbert Ziener, Heinz-Peter Schlemmer, Helmut Karl Seitz, Nina Waldburger, Thomas Longerich, Beate Katharina Straub, Sebastian Mueller
• E-Jahr: 2017
• Jahr: September 2017
• Umfang: 8 S.
• Fussnoten: Gesehen am 30.05.2018
• Titel Quelle: Enthalten in: Journal of hepatology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1985
• Jahr Quelle: 2017
• Band/Heft Quelle: 67(2017), 3, Seite 535-542
• ISSN Quelle: 1600-0641
• DOI: doi:10.1016/j.jhep.2017.04.019
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Alcoholic liver disease
• Sach-SW: Anemia
• Sach-SW: Atomic absorption spectroscopy
• Sach-SW: Cirrhosis
• Sach-SW: Hemochromatosis
• Sach-SW: Iron overload
• Sach-SW: Liver fibrosis
• Sach-SW: Liver iron
• Sach-SW: Liver stiffness
• Sach-SW: Magnetic susceptibility
• Sach-SW: Susceptometry
• K10plus-PPN: 1575844133

Abstract

Background & Aims Liver iron accumulates in various chronic liver diseases where it is an independent factor for survival and carcinogenesis. We tested a novel room-temperature susceptometer (RTS) to non-invasively assess liver iron concentration (LIC). Methods Two hundred and sixty-four patients with or without signs of iron overload or liver disease were prospectively enrolled. Thirty-five patients underwent liver biopsy with semiquantitative iron determination (Prussian Blue staining), atomic absorption spectroscopy (AAS, n=33), or magnetic resonance imaging (MRI, n=15). Results In vitro studies demonstrated a highly linear (r2=0.998) association between RTS-signal and iron concentration, with a detection limit of 0.3mM. Using an optimized algorithm, accounting for the skin-to-liver capsule distance, valid measurements could be obtained in 84% of cases. LIC-RTS showed a significant correlation with LIC-AAS (r=0.74, p<0.001), LIC-MRI (r=0.64, p<0.001) and hepatocellular iron (r=0.58, p<0.01), but not with macrophage iron (r=0.32, p=0.30). Normal LIC-RTS was 1.4mg/g dry weight. Besides hereditary and transfusional iron overload, LIC-RTS was also significantly elevated in patients with alcoholic liver disease. The areas under the receiver operating characteristic curve (AUROC) for grade 1, 2 and 3 hepatocellular iron overload were 0.72, 0.89 and 0.97, respectively, with cut-off values of 2.0, 4.0 and 5.0mg/g dry weight. Notably, the positive and negative predictive values, sensitivity, specificity and accuracy of severe hepatic iron overload (HIO) (grade ≥2) detection, were equal to AAS and superior to all serum iron markers. Depletion of hepatic iron could be efficiently monitored upon phlebotomy. Conclusions RTS allows for the rapid and non-invasive measurement of LIC. In comparison to MRI, it could be a cost-effective bedside method for LIC screening. Lay summary: Novel room-temperature susceptometer (RTS) allows for the rapid, sensitive, and non-invasive measurement of liver iron concentration. In comparison to MRI, it could be a cost-effective bedside method for liver iron concentration screening.