In vivo RNAi in the drosophila follicular epithelium

• Verfasst von: Riechmann, Veit [VerfasserIn]
• Titel: In vivo RNAi in the drosophila follicular epithelium
• Titelzusatz: analysis of stem cell maintenance, proliferation, and differentiation
• Verf.angabe: Veit Riechmann
• E-Jahr: 2017
• Jahr: 4 July 2017
• Umfang: 22 S.
• Fussnoten: Gesehen am 05.06.2018
• Titel Quelle: Enthalten in: RNAi and Small Regulatory RNAs in Stem Cells
• Ort Quelle: New York, NY : Humana Press, 2017
• Jahr Quelle: 2017
• Band/Heft Quelle: (2017), Seite 185-206
• ISBN Quelle: 978-1-4939-7108-4
• DOI: doi:10.1007/978-1-4939-7108-4_14
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1575974509

Abstract

In vivo RNAi in Drosophila facilitates simple and rapid analysis of gene functions in a cell- or tissue-specific manner. The versatility of the UAS-GAL4 system allows to control exactly where and when during development the function of a gene is depleted. The epithelium of the ovary is a particularly good model to study in a living animal how stem cells are maintained and how their descendants proliferate and differentiate. Here I provide basic information about the publicly available reagents for in vivo RNAi, and I describe how the oogenesis system can be applied to analyze stem cells and epithelial development at a histological level. Moreover, I give helpful hints to optimize the use of the UAS-GAL4 system for RNAi induction in the follicular epithelium. Finally, I provide detailed step-by-step protocols for ovary dissection, antibody stainings, and ovary mounting for microscopic analysis.