Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Gabel, Michael [VerfasserIn]   i
 Regös, Roland Robert [VerfasserIn]   i
 Graw, Frederik [VerfasserIn]   i
Titel:More or less - on the influence of labelling strategies to infer cell population dynamics
Verf.angabe:Michael Gabel, Roland R. Regoes, Frederik Graw
E-Jahr:2017
Jahr:October 18, 2017
Umfang:Illustrationen
 17 S.
Fussnoten:Gesehen am 06.06.2018
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2017
Band/Heft Quelle:12(2017,10), Artikelnummer e0185523, 17 Seiten
ISSN Quelle:1932-6203
Abstract:The adoptive transfer of labelled cell populations has been an essential tool to determine and quantify cellular dynamics. The experimental methods to label and track cells over time range from fluorescent dyes over congenic markers towards single-cell labelling techniques, such as genetic barcodes. While these methods have been widely used to quantify cell differentiation and division dynamics, the extent to which the applied labelling strategy actually affects the quantification of the dynamics has not been determined so far. This is especially important in situations where measurements can only be obtained at a single time point, as e.g. due to organ harvest. To this end, we studied the appropriateness of various labelling strategies as characterised by the number of different labels and the initial number of cells per label to quantify cellular dynamics. We simulated adoptive transfer experiments in systems of various complexity that assumed either homoeostatic cellular turnover or cell expansion dynamics involving various steps of cell differentiation and proliferation. Re-sampling cells at a single time point, we determined the ability of different labelling strategies to recover the underlying kinetics. Our results indicate that cell transition and expansion rates are differently affected by experimental shortcomings, such as loss of cells during transfer or sampling, dependent on the labelling strategy used. Furthermore, uniformly distributed labels in the transferred population generally lead to more robust and less biased results than non-equal label sizes. In addition, our analysis indicates that certain labelling approaches incorporate a systematic bias for the identification of complex cell expansion dynamics.
DOI:doi:10.1371/journal.pone.0185523
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: http://dx.doi.org/10.1371/journal.pone.0185523
 kostenfrei: Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0185523
 DOI: https://doi.org/10.1371/journal.pone.0185523
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Adoptive transfer
 Cell activation
 Cell death
 Cell differentiation
 Cell proliferation
 Population dynamics
 Precursor cells
 T cells
K10plus-PPN:1576026426
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68258225   QR-Code
zum Seitenanfang