Fluorescence nanoscopy in cell biology

• Verfasst von: Sahl, Steffen Joachim [VerfasserIn]
• Verfasst von: Hell, Stefan [VerfasserIn]
• Verfasst von: Jakobs, Stefan [VerfasserIn]
• Titel: Fluorescence nanoscopy in cell biology
• Verf.angabe: Steffen J. Sahl, Stefan W. Hell, Stefan Jakobs
• Jahr: 2017
• Umfang: 17 S.
• Fussnoten: Published online 6 Sep 2017 ; Gesehen am 12.06.2018
• Titel Quelle: Enthalten in: Nature reviews / Molecular cell biology
• Ort Quelle: London : Macmillan, 2000
• Jahr Quelle: 2017
• Band/Heft Quelle: 18(2017), 11, Seite 685-701
• ISSN Quelle: 1471-0080
• DOI: doi:10.1038/nrm.2017.71
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1576299414

Abstract

Fluorescence nanoscopy uniquely combines minimally invasive optical access to the internal nanoscale structure and dynamics of cells and tissues with molecular detection specificity. While the basic physical principles of 'super-resolution' imaging were discovered in the 1990s, with initial experimental demonstrations following in 2000, the broad application of super-resolution imaging to address cell-biological questions has only more recently emerged. Nanoscopy approaches have begun to facilitate discoveries in cell biology and to add new knowledge. One current direction for method improvement is the ambition to quantitatively account for each molecule under investigation and assess true molecular colocalization patterns via multi-colour analyses. In pursuing this goal, the labelling of individual molecules to enable their visualization has emerged as a central challenge. Extending nanoscale imaging into (sliced) tissue and whole-animal contexts is a further goal. In this Review we describe the successes to date and discuss current obstacles and possibilities for further development.