| |  Online-Ressource |
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| Verfasst von: | Sakin, Volkan [VerfasserIn]  |
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| | Hanne, Janina [VerfasserIn] |
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| | Dunder, Jessica [VerfasserIn] |
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| | Anders-Ößwein, Maria [VerfasserIn] |
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| | Laketa, Vibor [VerfasserIn] |
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| | Nikić-Spiegel, Ivana [VerfasserIn] |
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| | Kräusslich, Hans-Georg [VerfasserIn] |
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| | Lemke, Edward A. [VerfasserIn] |
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| | Müller, Barbara [VerfasserIn] |
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| Titel: | A versatile tool for live-cell imaging and super-resolution nanoscopy studies of HIV-1 env distribution and mobility |
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| Verf.angabe: | Volkan Sakin, Janina Hanne, Jessica Dunder, Maria Anders-Össwein, Vibor Laketa, Ivana Nikić, Hans-Georg Kräusslich, Edward A. Lemke, Barbara Müller |
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| Jahr: | 2017 |
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| Umfang: | 11 S. |
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| Teil: | volume:24 |
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| | year:2017 |
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| | number:5 |
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| | pages:635-645 |
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| | extent:11 |
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| Fussnoten: | Available online 29 April 2017 ; Gesehen am 18.06.2018 |
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| Titel Quelle: | Enthalten in: Cell chemical biology |
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| Ort Quelle: | Amsterdam : Elsevier, 2016 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 24(2017), 5, Seite 635-645 |
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| ISSN Quelle: | 2451-9448 |
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| Abstract: | Summary: The envelope glycoproteins (Env) of HIV-1 mediate cell entry through fusion of the viral envelope with a target cell membrane. Intramembrane mobility and clustering of Env trimers at the viral budding site are essential for its function. Previous live-cell and super-resolution microscopy studies were limited by lack of a functional fluorescent Env derivative, requiring antibody labeling for detection. Introduction of a bio-orthogonal amino acid by genetic code expansion, combined with click chemistry, offers novel possibilities for site-specific, minimally invasive labeling. Using this approach, we established efficient incorporation of non-canonical amino acids within HIV-1 Env in mammalian cells. The engineered protein retained plasma membrane localization, glycosylation, virion incorporation, and fusogenic activity, and could be rapidly and specifically labeled with synthetic dyes. This strategy allowed us to revisit Env dynamics and nanoscale distribution at the plasma membrane close to its native state, applying fluorescence recovery after photo bleaching and STED nanoscopy, respectively. |
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| DOI: | doi:10.1016/j.chembiol.2017.04.007 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.chembiol.2017.04.007 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S2451945617301071 |
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| | DOI: https://doi.org/10.1016/j.chembiol.2017.04.007 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | amber suppression |
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| | click labeling |
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| | envelope protein, gp120 |
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| | fluorescence recovery after photo bleaching |
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| | genetic code expansion |
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| | human immunodeficiency virus |
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| | non-canonical amino acids |
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| | STED nanoscopy |
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| K10plus-PPN: | 1576450058 |
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| Verknüpfungen: | → Zeitschrift |
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¬A¬ versatile tool for live-cell imaging and super-resolution nanoscopy studies of HIV-1 env distribution and mobility / Sakin, Volkan [VerfasserIn]; 2017 (Online-Ressource)
