| Online-Ressource |
Verfasst von: | Suzuki, Yosuke [VerfasserIn]  |
| Witt, Lukas [VerfasserIn]  |
| Mier, Walter [VerfasserIn]  |
| Mikus, Gerd [VerfasserIn]  |
| Markert, Christoph [VerfasserIn]  |
| Haefeli, Walter E. [VerfasserIn]  |
| Burhenne, Jürgen [VerfasserIn]  |
Titel: | Ultra-sensitive and selective quantification of endothelin-1 in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry |
Verf.angabe: | Yosuke Suzuki, Lukas Witt, Walter Mier, Gerd Mikus, Christoph Markert, Walter E. Haefeli, Jürgen Burhenne |
Umfang: | 7 S. |
Fussnoten: | Available online 29 April 2017 ; Gesehen am 20.06.2018 |
Titel Quelle: | Enthalten in: Journal of pharmaceutical and biomedical analysis |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 142(2017), S. 84-90 |
ISSN Quelle: | 1873-264X |
Abstract: | Endothelin-1 (ET-1) is a potent endogenous vasoconstrictor peptide and the plasma concentrations are commonly quantified by immunoassays such as enzyme-linked immuno-sorbent assays (ELISA) with the disadvantage of possible cross-reactivity with closely related endothelin derivatives. The aim of this study was to develop and validate an ultra-sensitive and selective assay for the quantification of ET-1 in human plasma, using ultra-performance liquid chromatography with tandem mass spectrometry (UPLC-MS/MS) after solid phase extraction. The assay fulfilled the requirements of the US Food and Drug Administration (FDA) and the European Medicines Agency (EMA) guidelines for assay validation, with a lower limit of quantification of 1.5pg/mL for ET-1. Recovery rates from plasma ranged between 80.8% and 93.6%, and matrix effect varied between 121% and 135%. The assay was successfully applied to assess the time course of plasma ET-1 concentrations in two human volunteers after co-administration of bosentan and clarithromycin. In this trial, the concentrations measured by UPLC-MS/MS were slightly lower than those measured by ELISA, with a strong positive correlation between the two methods. Our novel UPLC-MS/MS method is applicable to the clinical setting and may have better selectivity for ET-1 than ELISA. |
DOI: | doi:10.1016/j.jpba.2017.04.038 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Verlag: http://dx.doi.org/10.1016/j.jpba.2017.04.038 |
| Verlag: http://www.sciencedirect.com/science/article/pii/S0731708517305319 |
| DOI: https://doi.org/10.1016/j.jpba.2017.04.038 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 1576580636 |
Verknüpfungen: | → Zeitschrift |
Ultra-sensitive and selective quantification of endothelin-1 in human plasma using ultra-performance liquid chromatography coupled to tandem mass spectrometry / Suzuki, Yosuke [VerfasserIn] (Online-Ressource)