Sex-dependent differences in the in vivo respiratory phenotype of the TASK-1 potassium channel knockout mouse

• Verfasst von: Jungbauer, Stefan [VerfasserIn]
• Verfasst von: Heitzmann, Dirk [VerfasserIn]
• Titel: Sex-dependent differences in the in vivo respiratory phenotype of the TASK-1 potassium channel knockout mouse
• Verf.angabe: Stefan Jungbauer, Philipp Karl Buehler, Jacqueline Neubauer, Cordula Haas, Dirk Heitzmann, Ines Tegtmeier, Christina Sterner, Jacques Barhanin, Michael Georgieff, Richard Warth, Jörg Thomas
• Jahr: 2017
• Jahr des Originals: 2016
• Umfang: 16 S.
• Fussnoten: Gesehen am 21.06.2018 ; Available online 10 November 2016
• Titel Quelle: Enthalten in: Respiratory physiology & neurobiology
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 2002
• Jahr Quelle: 2017
• Band/Heft Quelle: 245(2017), Seite 13-28
• ISSN Quelle: 1878-1519
• DOI: doi:10.1016/j.resp.2016.11.005
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Chemoreception
• Sach-SW: Neonatal mice
• Sach-SW: Sex-dependent
• Sach-SW: TASK-1 channel
• Sach-SW: Whole body plethysmograph
• K10plus-PPN: 157674258X

Abstract

TASK-1 potassium channels have been implicated in central and peripheral chemoreception; however, the precise contribution of TASK-1 for the control of respiration is still under debate. Here, we investigated the respiration of unrestrained adult and neonatal TASK-1 knockout mice (TASK-1−/−) using a plethysmographic device. Respiration in adult female TASK-1−/− mice under control (21% O2), hypoxia and hypercapnia was unaffected. Under acute hypoxia male TASK-1−/− mice exhibited a reduced increase of the respiratory frequency (fR) compared to wildtypes. However, the tidal volume (VT) of male TASK-1−/− mice was strongly enhanced. The volatile anesthetic isoflurane induced in male TASK-1−/− and male wild type mice (TASK-1+/+) a similar respiratory depression. Neonatal TASK-1−/− mice demonstrated a 30-40% decrease of the minute volume, caused by a reduction of the fR under control condition (21% O2). Under hypoxia, neonatal TASK-1−/− mice more frequently stopped breathing (apnea>3s) suggesting an increased hypoxia-sensitivity. As reported before, this increased hypoxia sensitivity had no influence on the survival rate of neonatal TASK-1−/− mice. In adult and neonatal mice, TASK-1 gene deletion induced a significant prolongation of the relaxation time (RT), which is a parameter for expiration kinetics. Additionally, screening for mutations in the human TASK-1 gene in 155 cases of sudden infant death syndrome (SIDS) was inconclusive. In conclusion, these data are suggestive for an increased hypoxia-sensitivity of neonatal TASK-1−/− mice, however, without causing an increase in neonatal lethality. In adult female TASK-1−/− mice respiration was unaffected, whereas adult male TASK-1−/− mice showed a modified breathing pattern. These results are suggestive for sex-specific mechanisms for compensating the inactivation of TASK-1 in mice.