Catalytic peroxynitrite decomposition improves reperfusion injury after heart transplantation

• Verfasst von: Szabó, Gábor [VerfasserIn]
• Verfasst von: Loganathan, Sivakkanan [VerfasserIn]
• Verfasst von: Karck, Matthias [VerfasserIn]
• Verfasst von: Radovits, Tamás [VerfasserIn]
• Titel: Catalytic peroxynitrite decomposition improves reperfusion injury after heart transplantation
• Verf.angabe: Gábor Szabó, Sivakkanan Loganathan, Béla Merkely, John T. Groves, Matthias Karck, Csaba Szabó and Tamás Radovits
• Umfang: 7 S.
• Fussnoten: Available online 6 March 2012 ; Gesehen am 22.06.2018
• Titel Quelle: Enthalten in: The journal of thoracic and cardiovascular surgery
• Jahr Quelle: 2012
• Band/Heft Quelle: 143(2012), 6, S. 1443-1449
• ISSN Quelle: 1097-685X
• DOI: doi:10.1016/j.jtcvs.2012.02.008
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1576764273

Abstract

Objective - Peroxynitrite, a reactive nitrogen species, has been implicated in the development of ischemia-reperfusion injury. The present study investigated the effects of the potent peroxynitrite decomposition catalyst FP15 on myocardial and endothelial function after hypothermic ischemia-reperfusion in a heterotopic rat heart transplantation model. - Methods - After a 1-hour ischemic preservation and implantation of donor hearts, reperfusion was started after application of vehicle (5% glucose solution) or FP15 (0.3 mg/kg). The assessment of left ventricular pressure-volume relations, total coronary blood flow, endothelial function, immunohistochemical markers of nitro-oxidative stress, and myocardial high-energy phosphates was performed at 1 and 24 hours of reperfusion. - Results - After 1 hour of reperfusion, myocardial contractility (maximal slope of systolic pressure increment at 140 μL left ventricular volume: 5435 ± 508 mm Hg/s vs 2346 ± 263 mm Hg/s), coronary blood flow (3.98 ± 0.33 mL/min/g vs 2.74 ± 0.29 mL/min/g), and endothelial function were significantly improved, nitro-oxidative stress was reduced, and myocardial high-energy phosphate content was preserved in the FP15-treated animals compared with controls. - Conclusions - Pharmacologic peroxynitrite decomposition reduces reperfusion injury after heart transplantation as the result of reduction of nitro-oxidative stress and prevention of energy depletion and exerts a beneficial effect against reperfusion-induced graft cardiac and coronary endothelial dysfunction.