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Verfasst von:Jäschke, Anja [VerfasserIn]   i
 Bujard, Hermann [VerfasserIn]   i
 Epp, Christian [VerfasserIn]   i
Titel:Merozoite surface protein 1 from plasmodium falciparum is a major target of opsonizing antibodies in individuals with acquired immunity against malaria
Verf.angabe:Anja Jäschke, Boubacar Coulibaly, Edmond J. Remarque, Hermann Bujard, Christian Epp
Jahr:2017
Umfang:16 S.
Fussnoten:Accepted manuscript posted online 6 September 2017 ; Gesehen am 26.06.2018
Titel Quelle:Enthalten in: Clinical and vaccine immunology
Ort Quelle:Washington, DC : American Society for Microbiology, 1994
Jahr Quelle:2017
Band/Heft Quelle:24(2017,1) Artikel-Nummer e00155-17, 16 Seiten
ISSN Quelle:1098-6588
 1556-679X
Abstract:Naturally acquired immunity against malaria is largely mediated by serum antibodies controlling levels of blood-stage parasites. A limited understanding of the antigenic targets and functional mechanisms of protective antibodies has hampered the development of efficient malaria vaccines. Besides directly inhibiting the growth of Plasmodium parasites, antibodies can opsonize merozoites and recruit immune effector cells such as monocytes and neutrophils. Antibodies against the vaccine candidate merozoite surface protein 1 (MSP-1) are acquired during natural infections and have been associated with protection against malaria in several epidemiological studies. Here we analyzed serum antibodies from semi-immune individuals from Burkina Faso for their potential (i) to directly inhibit the growth of P. falciparum blood stages in vitro and (ii) to opsonize merozoites and to induce the antibody-dependent respiratory burst (ADRB) activity of neutrophils. While a few sera that directly inhibited the growth of P. falciparum blood stages were identified, immunoglobulin G (IgG) from all individuals clearly mediated the activation of neutrophils. The level of neutrophil activation correlated with levels of antibodies to MSP-1, and affinity-purified MSP-1-specific antibodies elicited ADRB activity. Furthermore, immunization of nonhuman primates with recombinant full-size MSP-1 induced antibodies that efficiently opsonized P. falciparum merozoites. Reversing the function by preincubation with recombinant antigens allowed us to quantify the contribution of MSP-1 to the antiparasitic effect of serum antibodies. Our data suggest that MSP-1, especially the partially conserved subunit MSP-183, is a major target of opsonizing antibodies acquired during natural exposure to malaria. Induction of opsonizing antibodies might be a crucial effector mechanism for MSP-1-based malaria vaccines.
DOI:doi:10.1128/CVI.00155-17
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1128/CVI.00155-17
 Kostenfrei: Volltext: http://cvi.asm.org/content/24/11/e00155-17
 DOI: https://doi.org/10.1128/CVI.00155-17
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:ADRB
 Neutrophil
 Opsonizing antibodies
 P. falciparum MSP-1
 Respiratory burst
K10plus-PPN:1576839109
Verknüpfungen:→ Zeitschrift

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