Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina

• Verfasst von: Chen, Fei [VerfasserIn]
• Verfasst von: Jonas, Jost B. [VerfasserIn]
• Titel: Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-D-aspartate-induced excitotoxicity in the adult rat retina
• Verf.angabe: Fei Chen, Libin Jiang, Ceying Shen, Hongfei Wan, Liang Xu, Ningli Wang, Jost B. Jonas
• Jahr: 2012
• Umfang: 7 S.
• Fussnoten: First published: 13 September 2012 ; Gesehen am 26.06.2018
• Titel Quelle: Enthalten in: Acta ophthalmologica
• Ort Quelle: Oxford : Blackwell, 2008
• Jahr Quelle: 2012
• Band/Heft Quelle: 90(2012), 8, Seite e609-e615
• ISSN Quelle: 1755-3768
• DOI: doi:10.1111/j.1755-3768.2012.02502.x
• Datenträger: Online-Ressource
• Sprache: eng
• Bibliogr. Hinweis: Erscheint auch als : Druck-Ausgabe: Neuroprotective effect of epigallocatechin-3-gallate against N-methyl-d-aspartate-induced excitotoxicity in the adult rat retina. - 2012
• Sach-SW: epigallocatechin-3-gallate
• Sach-SW: excitotoxicity
• Sach-SW: glaucoma
• Sach-SW: N-methyl-d-aspartate
• Sach-SW: neuroprotection
• Sach-SW: optic nerve
• K10plus-PPN: 1576857603

Abstract

Abstract. Purpose: Epigallocatechin-3-gallate (EGCG), the major polyphenol of green tea, has been suggested to reduce glutamate excitotoxicity. We therefore investigated the potentially protective effects of EGCG against N-methyl-d-aspartate (NMDA)-induced excitotoxicity in the retina. Methods: Female Wistar rats (n = 171) were divided into a normal control group (n = 9); saline control group with intravitreal saline injections (n = 54); NMDA control group with an intravitreal NMDA injection and intraperitoneal saline injections (n = 54); and NMDA study group (n = 54) receiving an intravitreal NMDA injection plus intraperitoneal EGCG (25 mg/kg) injections. Starting at 2 days prior to the intravitreal NMDA injection, the intraperitoneal injections were performed daily for the whole study period. At 12 hr, 1, 2, 3 days, 1 and 2 weeks after the intravitreal NMDA injection, the animals were killed. We counted the neurons in the retinal ganglion cell layer (GCL) on histological sections, measured the thickness of Thy-1 immunoreactivity and assessed the expression of Thy-1 mRNA by real-time polymerase chain reaction. Results: At all time-points, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA were significantly (all p < 0.05) lower in the NMDA control group than in the NMDA study group, in which the parameters were significantly (all p < 0.05) lower than in the saline control group and the normal control group. In both groups with an intravitreal NMDA injection, GCL cell density, thickness of Thy-1 immunoreactivity and expression of Thy-1 mRNA decreased significantly with increasing follow-up time. Conclusions: Intraperitoneal application of EGCG resulted in a significantly less marked NMDA-associated loss of retinal ganglion cells.