Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
Verfasst von:Hoffmann, Sandra [VerfasserIn]   i
 Schmitteckert, Stefanie [VerfasserIn]   i
 Griesbeck, Anne [VerfasserIn]   i
 Preiss, Hannes [VerfasserIn]   i
 Sumer, Simon [VerfasserIn]   i
 Granzow, Martin [VerfasserIn]   i
 Eckstein, Volker [VerfasserIn]   i
 Niesler, Beate [VerfasserIn]   i
 Rappold, Gudrun [VerfasserIn]   i
Titel:Comparative expression analysis of Shox2-deficient embryonic stem cell-derived sinoatrial node-like cells
Verf.angabe:Sandra Hoffmann, Stefanie Schmitteckert, Anne Griesbeck, Hannes Preiss, Simon Sumer, Alexandra Rolletschek, Martin Granzow, Volker Eckstein, Beate Niesler, Gudrun A. Rappold
Umfang:7 S.
Fussnoten:Available online 29 March 2017 ; Gesehen am 27.06.2018
Titel Quelle:Enthalten in: Stem cell research
Jahr Quelle:2017
Band/Heft Quelle:21(2017), S. 51-57
ISSN Quelle:1876-7753
Abstract:The homeodomain transcription factor Shox2 controls the development and function of the native cardiac pacemaker, the sinoatrial node (SAN). Moreover, SHOX2 mutations have been associated with cardiac arrhythmias in humans. For detailed examination of Shox2-dependent developmental mechanisms in SAN cells, we established a murine embryonic stem cell (ESC)-based model using Shox2 as a molecular tool. Shox2+/+ and Shox2−/− ESC clones were isolated and differentiated according to five different protocols in order to evaluate the most efficient enrichment of SAN-like cells. Expression analysis of cell subtype-specific marker genes revealed most efficient enrichment after CD166-based cell sorting. Comparative cardiac expression profiles of Shox2+/+ and Shox2−/− ESCs were examined by nCounter technology. Among other genes, we identified Nppb as a novel putative Shox2 target during differentiation in ESCs. Differential expression of Nppb could be confirmed in heart tissue of Shox2−/− embryos. Taken together, we established an ESC-based cardiac differentiation model and successfully purified Shox2+/+ and Shox2−/− SAN-like cells. This now provides an excellent basis for the investigation of molecular mechanisms under physiological and pathophysiological conditions for evaluating novel therapeutic approaches.
DOI:doi:10.1016/j.scr.2017.03.018
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1016/j.scr.2017.03.018
 Verlag: http://www.sciencedirect.com/science/article/pii/S1873506117300624
 DOI: https://doi.org/10.1016/j.scr.2017.03.018
Sprache:eng
K10plus-PPN:157690556X
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68274788   QR-Code
zum Seitenanfang