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Verfasst von:Tucher, Christine [VerfasserIn]   i
 Bode, Konrad A. [VerfasserIn]   i
 Schiller, Petra [VerfasserIn]   i
 Claßen, Laura [VerfasserIn]   i
 Birr, Carolin [VerfasserIn]   i
 Souto-Carneiro, Maria Margarida [VerfasserIn]   i
 Blank, Norbert [VerfasserIn]   i
 Lorenz, Hanns-Martin [VerfasserIn]   i
 Schiller, Martin [VerfasserIn]   i
Titel:Extracellular vesicle subtypes released from activated or apoptotic T-lymphocytes carry a specific and stimulus-dependent protein cargo
Verf.angabe:Christine Tucher, Konrad Bode, Petra Schiller, Laura Claßen, Carolin Birr, Maria Margarida Souto-Carneiro, Norbert Blank, Hanns-Martin Lorenz and Martin Schiller
E-Jahr:2018
Jahr:15 March 2018
Teil:volume:9
 year:2018
Fussnoten:Gesehen am 03.07.2018
Titel Quelle:Enthalten in: Frontiers in immunology
Ort Quelle:Lausanne : Frontiers Media, 2010
Jahr Quelle:2018
Band/Heft Quelle:9(2018) Artikel-Nummer 534, 13 Seiten
ISSN Quelle:1664-3224
Abstract:Extracellular vesicles (EVs) are released from nearly all mammalian cells and different EV populations have been described. Microvesicles represent large EVs (LEVs) released from the cellular surface, while exosomes are small EVs (SEVs) released from an intracellular compartment. As it is likely that different stimuli promote the release of distinct EV populations, we analyzed EVs from human lymphocytes considering the respective release stimuli (activation vs. apoptosis induction). We could clearly separate two EV populations, namely SEVs (average diameter <200nm) and LEVs (diameter range between 200 and 1000nm). Morphology and size were analyzed by electron microscopy and nanoparticle tracking analysis. Apoptosis induction caused a massive release of LEVs, while activated T cells released SEVs and LEVs in considerably lower amounts. The release of SEVs from apoptotic T-cells was comparable to LEV release from activated ones. LEVs contained signaling proteins and proteins of the actin-myosin cytoskeleton. SEVs carried cytoplasmic/endosomal proteins like the 70-kDa heat shock protein (HSP70) or tumor susceptibility 101 (TSG101), microtubule-associated proteins, and ubiquitinated proteins. The protein expression profile of SEVs and LEVs changed substantially after the induction of apoptosis. After apoptosis induction HSP70 and TSG101 (often used as exosome markers) were highly expressed within LEVs. Interestingly, in contrast to HSP70 and TSG101, gelsolin and eps15 homology domain-containing protein 3 (EHD3) turned out to be specific for SEVs irrespective of the stimulus causing the EV release. Finally, we detected several subunits of the proteasome (PSMB9, PSMB10) as well as the danger signal HMGB1 exclusively within apoptotic cell-released LEVs. Thus, we were able to identify new marker proteins that can be useful to discriminate between distinct LEV subpopulations.
DOI:doi:10.3389/fimmu.2018.00534
URL:Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.3389/fimmu.2018.00534
 Kostenfrei: Volltext: https://www.frontiersin.org/articles/10.3389/fimmu.2018.00534/full
 DOI: https://doi.org/10.3389/fimmu.2018.00534
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Apoptosis
 Exosomes
 extracellular vesicles
 Microvesicles
 stimulus dependent release
K10plus-PPN:1577237242
Verknüpfungen:→ Zeitschrift
 
 
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