| Online-Ressource |
Verfasst von: | Müller, Irene [VerfasserIn]  |
| Rohde, David [VerfasserIn]  |
| Most, Patrick [VerfasserIn]  |
Titel: | Pathogenic role of the damage-associated molecular patterns S100A8 and S100A9 in coxsackievirus B3-induced myocarditis |
Verf.angabe: | Irene Müller, Thomas Vogl, Kathleen Pappritz, Kapka Miteva, Konstantinos Savvatis, David Rohde, Patrick Most, Dirk Lassner, Burkert Pieske, Uwe Kühl, Sophie Van Linthout, Carsten Tschöpe |
Fussnoten: | Gesehen am 04.07.2018 |
Titel Quelle: | Enthalten in: Circulation / Heart failure |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 10(2017,11) Artikel-Nummer e004125, 31 Seiten |
ISSN Quelle: | 1941-3297 |
Abstract: | Background: The alarmins S100A8 and S100A9 are damage-associated molecular patterns, which play a pivotal role in cardiovascular diseases, inflammation, and viral infections. We aimed to investigate their role in Coxsackievirus B3 (CVB3)-induced myocarditis. Methods and Results: S100A8 and S100A9 mRNA expression was 13.0-fold (P=0.012) and 5.1-fold (P=0.038) higher in endomyocardial biopsies from patients with CVB3-positive myocarditis compared with controls, respectively. Elimination of CVB3 led to a downregulation of these alarmins. CVB3-infected mice developed an impaired left ventricular function and displayed an increased left ventricular S100A8 and S100A9 protein expression versus controls. In contrast, CVB3-infected S100A9 knockout mice, which are also a complete knockout for S100A8 on protein level, showed an improved left ventricular function, which was associated with a reduced cardiac inflammatory and oxidative response, and lower CVB3 copy number compared with wild-type CVB3 mice. Exogenous application of S100A8 to S100A9 knockout CVB3 mice induced a severe myocarditis similar to wild-type CVB3 mice. In CVB3-infected HL-1 cells, S100A8 and S100A9 enhanced oxidative stress and CVB3 copy number compared with unstimulated infected cells. In CVB3-infected RAW macrophages, both alarmins increased MIP-2 (macrophage inflammatory protein-2) chemokine expression, which was reduced in CVB3 S100A8 knockdown versus scrambled siRNA CVB3 cells. Conclusions: S100A8 and S100A9 aggravate CVB3-induced myocarditis and might serve as therapeutic targets in inflammatory cardiomyopathies. |
DOI: | doi:10.1161/CIRCHEARTFAILURE.117.004125 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Teilw. Kostenfrei: Verlag: http://dx.doi.org/10.1161/CIRCHEARTFAILURE.117.004125 |
| Teilw. Kostenfrei: Verlag: http://circheartfailure.ahajournals.org.ezproxy.medma.uni-heidelberg.de/content/10/11/e004125 |
| DOI: https://doi.org/10.1161/CIRCHEARTFAILURE.117.004125 |
Datenträger: | Online-Ressource |
Sprache: | eng |
K10plus-PPN: | 157726018X |
Verknüpfungen: | → Zeitschrift |
Pathogenic role of the damage-associated molecular patterns S100A8 and S100A9 in coxsackievirus B3-induced myocarditis / Müller, Irene [VerfasserIn] (Online-Ressource)