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Status: Bibliographieeintrag

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Verfasst von:Buffington, Shelly Alexandra [VerfasserIn]   i
 Sobotzik, Jürgen-Markus [VerfasserIn]   i
 Schultz, Christian [VerfasserIn]   i
Titel:[Iota][Kappa]Bα is not required for axon initial segment assembly
Verf.angabe:Shelly A. Buffington, Jürgen M. Sobotzik, Christian Schultz, Matthew N. Rasband
E-Jahr:2012
Jahr:15 March 2012
Umfang:9 S.
Fussnoten:Available online 15 March 2012 ; Gesehen am 05.07.2018
Titel Quelle:Enthalten in: Molecular and cellular neuroscience
Ort Quelle:San Diego, Calif. : Elsevier, 1990
Jahr Quelle:2012
Band/Heft Quelle:50(2012), 1, Seite 1-9
ISSN Quelle:1095-9327
Abstract:The inhibitor of NF-κB alpha (IκBα) protein is an important regulator of the transcription factor NF-κB. In neurons, IκBα has been shown to play a role in neurite outgrowth and cell survival. Recently, a phosphorylated form of IκBα (pIκBα Ser32/36) was reported to be highly enriched at the axon initial segment (AIS) and was proposed to function upstream of ankyrinG in AIS assembly, including ion channel recruitment. However, we report here that the AIS clustering of ankyrinG and Na+ channels in the brains of IκBα knockout (Nfkbia−/−) mice is comparable to that in wild-type littermates. Furthermore, we found that multiple phospho-specific antibodies against pIκBα Ser32/36 non-specifically label AIS in Nfkbia−/− cortex and AIS in dissociated Nfkbia−/− hippocampal neurons. With the exception of ankyrinG, shRNA-mediated knockdown of known AIS proteins in cultured hippocampal neurons did not eliminate the AIS labeling with pIκBα antibodies. Instead, the pIκBα antibodies cross-react with a phosphorylated epitope of a protein associated with the microtubule-based AIS cytoskeleton that is not integrated into the AIS membrane complex organized by ankyrinG. Our results indicate that pIκBα is neither enriched at the AIS nor required for AIS assembly.
DOI:doi:10.1016/j.mcn.2012.03.003
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1016/j.mcn.2012.03.003
 Volltext: http://www.sciencedirect.com/science/article/pii/S1044743112000437
 DOI: https://doi.org/10.1016/j.mcn.2012.03.003
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:AnkyrinG
 Axon initial segment
 Neuronal polarity
 NF-κB signaling pathway
K10plus-PPN:157731722X
Verknüpfungen:→ Zeitschrift

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