Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Buffington, Shelly Alexandra [VerfasserIn]  |
| Sobotzik, Jürgen-Markus [VerfasserIn]  |
| Schultz, Christian [VerfasserIn]  |
Titel: | [Iota][Kappa]Bα is not required for axon initial segment assembly |
Verf.angabe: | Shelly A. Buffington, Jürgen M. Sobotzik, Christian Schultz, Matthew N. Rasband |
E-Jahr: | 2012 |
Jahr: | 15 March 2012 |
Umfang: | 9 S. |
Fussnoten: | Available online 15 March 2012 ; Gesehen am 05.07.2018 |
Titel Quelle: | Enthalten in: Molecular and cellular neuroscience |
Ort Quelle: | San Diego, Calif. : Elsevier, 1990 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | 50(2012), 1, Seite 1-9 |
ISSN Quelle: | 1095-9327 |
Abstract: | The inhibitor of NF-κB alpha (IκBα) protein is an important regulator of the transcription factor NF-κB. In neurons, IκBα has been shown to play a role in neurite outgrowth and cell survival. Recently, a phosphorylated form of IκBα (pIκBα Ser32/36) was reported to be highly enriched at the axon initial segment (AIS) and was proposed to function upstream of ankyrinG in AIS assembly, including ion channel recruitment. However, we report here that the AIS clustering of ankyrinG and Na+ channels in the brains of IκBα knockout (Nfkbia−/−) mice is comparable to that in wild-type littermates. Furthermore, we found that multiple phospho-specific antibodies against pIκBα Ser32/36 non-specifically label AIS in Nfkbia−/− cortex and AIS in dissociated Nfkbia−/− hippocampal neurons. With the exception of ankyrinG, shRNA-mediated knockdown of known AIS proteins in cultured hippocampal neurons did not eliminate the AIS labeling with pIκBα antibodies. Instead, the pIκBα antibodies cross-react with a phosphorylated epitope of a protein associated with the microtubule-based AIS cytoskeleton that is not integrated into the AIS membrane complex organized by ankyrinG. Our results indicate that pIκBα is neither enriched at the AIS nor required for AIS assembly. |
DOI: | doi:10.1016/j.mcn.2012.03.003 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1016/j.mcn.2012.03.003 |
| Volltext: http://www.sciencedirect.com/science/article/pii/S1044743112000437 |
| DOI: https://doi.org/10.1016/j.mcn.2012.03.003 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | AnkyrinG |
| Axon initial segment |
| Neuronal polarity |
| NF-κB signaling pathway |
K10plus-PPN: | 157731722X |
Verknüpfungen: | → Zeitschrift |
[Iota][Kappa]Bα is not required for axon initial segment assembly / Buffington, Shelly Alexandra [VerfasserIn]; 15 March 2012 (Online-Ressource)
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