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Status: Bibliographieeintrag

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Verfasst von:Waadt, Rainer [VerfasserIn]   i
 Krebs, Melanie [VerfasserIn]   i
 Schumacher, Karin [VerfasserIn]   i
Titel:Multiparameter imaging of calcium and abscisic acid and high-resolution quantitative calcium measurements using R-GECO1-mTurquoise in Arabidopsis
Verf.angabe:Rainer Waadt, Melanie Krebs, Jörg Kudla and Karin Schumacher
Umfang:18 S.
Fussnoten:Gesehen am 11.07.2018
Titel Quelle:Enthalten in: The new phytologist
Jahr Quelle:2017
Band/Heft Quelle:216(2017), 1, S. 303-320
ISSN Quelle:1469-8137
Abstract:Calcium signals occur in specific spatio-temporal patterns in response to various stimuli and are coordinated with, for example, hormonal signals, for physiological and developmental adaptations. Quantification of calcium together with other signalling molecules is required for correlative analyses and to decipher downstream calcium-decoding mechanisms. Simultaneous in vivo imaging of calcium and abscisic acid has been performed here to investigate the interdependence of the respective signalling processes in Arabidopsis thaliana roots. Advanced ratiometric genetically encoded calcium indicators have been generated and in vivo calcium calibration protocols were established to determine absolute calcium concentration changes in response to auxin and ATP. In roots, abscisic acid induced long-term basal calcium concentration increases, while auxin triggered rapid signals in the elongation zone. The advanced ratiometric calcium indicator R-GECO1-mTurquoise exhibited an increased calcium signal resolution compared to commonly used Förster resonance energy transfer-based indicators. Quantitative calcium measurements in Arabidopsis root tips using R-GECO1-mTurquoise revealed detailed maps of absolute calcium concentration changes in response to auxin and ATP. Calcium calibration protocols using R-GECO1-mTurquoise enabled high-resolution quantitative imaging of resting cytosolic calcium concentrations and their dynamic changes that revealed distinct hormonal and ATP responses in roots.
DOI:doi:10.1111/nph.14706
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Verlag: http://dx.doi.org/10.1111/nph.14706
 Verlag: https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.14706
 DOI: https://doi.org/10.1111/nph.14706
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1577484134
Verknüpfungen:→ Zeitschrift

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