| |  Online-Ressource |
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| Verfasst von: | Wünsche, Peer [VerfasserIn]  |
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| | Eckert, Elias Simon Peter [VerfasserIn] |
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| | Fronza, Raffaele [VerfasserIn] |
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| | Gil-Fariña, Irene [VerfasserIn] |
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| | Schmidt, Manfred [VerfasserIn] |
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| | Kalle, Christof von [VerfasserIn] |
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| | Herbst, Friederike [VerfasserIn] |
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| | Glimm, Hanno [VerfasserIn] |
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| Titel: | Mapping active gene-regulatory regions in human repopulating long-term HSCs |
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| Verf.angabe: | Peer Wünsche, Elias S.P. Eckert, Tim Holland-Letz, Anna Paruzynski, Agnes Hotz-Wagenblatt, Raffaele Fronza, Tim Rath, Irene Gil-Farina, Manfred Schmidt, Christof von Kalle, Christoph Klein, Claudia R. Ball, Friederike Herbst, Hanno Glimm |
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| E-Jahr: | 2018 |
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| Jahr: | 5 July 2018 |
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| Umfang: | 15 S. |
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| Fussnoten: | Gesehen am 11.07.2018 |
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| Titel Quelle: | Enthalten in: Cell stem cell |
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| Ort Quelle: | Amsterdam [u.a.] : Elsevier, 2007 |
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| Jahr Quelle: | 2018 |
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| Band/Heft Quelle: | 23(2018), 1, Seite 132-146 |
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| ISSN Quelle: | 1875-9777 |
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| Abstract: | Summary Genes that regulate hematopoietic stem cell (HSC) self-renewal, proliferation, and differentiation are tightly controlled by regulatory regions. However, mapping such regions relies on surface markers and immunophenotypic definition of HSCs. Here, we use γ-retroviral integration sites (γRV ISs) from a gene therapy trial for 10 patients with Wiskott-Aldrich syndrome to mark active enhancers and promoters in functionally defined long-term repopulating HSCs. Integration site clusters showed the highest ATAC-seq signals at HSC-specific peaks and strongly correlated with hematopoietic risk variants. Tagged genes were significantly enriched for HSC gene sets. We were able to map over 3,000 HSC regulatory regions in late-contributing HSCs, and we used these data to identify miR-10a and miR-335 as two miRNAs regulating early hematopoiesis. In this study, we show that viral insertion sites can be used as molecular tags to assess chromatin conformation on functionally defined cell populations, thereby providing a genome-wide resource for regulatory regions in human repopulating long-term HSCs. |
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| DOI: | doi:10.1016/j.stem.2018.06.003 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext ; Verlag: http://dx.doi.org/10.1016/j.stem.2018.06.003 |
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| | Volltext: http://www.sciencedirect.com/science/article/pii/S1934590918302856 |
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| | DOI: https://doi.org/10.1016/j.stem.2018.06.003 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | enhancer |
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| | hematopoietic stem cell |
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| | HSC |
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| | human |
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| | miRNA-10a |
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| | miRNA-335 |
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| | non-coding regulatory regions |
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| | viral insertion sites |
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| K10plus-PPN: | 1577488210 |
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| Verknüpfungen: | → Zeitschrift |
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Mapping active gene-regulatory regions in human repopulating long-term HSCs / Wünsche, Peer [VerfasserIn]; 5 July 2018 (Online-Ressource)
