Rapid detection of ampicillin resistance in Escherichia coli by quantitative mass spectrometry

• Verfasst von: Grundt, Alexander [VerfasserIn]
• Verfasst von: Findeisen, Peter [VerfasserIn]
• Verfasst von: Miethke, Thomas [VerfasserIn]
• Verfasst von: Jäger, Evelyn [VerfasserIn]
• Verfasst von: Ahmad-Nejad, Parviz [VerfasserIn]
• Verfasst von: Neumaier, Michael [VerfasserIn]
• Titel: Rapid detection of ampicillin resistance in Escherichia coli by quantitative mass spectrometry
• Verf.angabe: A. Grundt, P. Findeisen, T. Miethke, E. Jäger, P. Ahmad-Nejad and M. Neumaier
• E-Jahr: 2012
• Jahr: May 2012
• Umfang: 3 S.
• Fussnoten: Gesehen am 16.07.2018
• Titel Quelle: Enthalten in: Journal of clinical microbiology
• Ort Quelle: Washington, DC : Soc., 1975
• Jahr Quelle: 2012
• Band/Heft Quelle: 50(2012), 5, Seite 1727-1729
• ISSN Quelle: 1098-660X
• DOI: doi:10.1128/JCM.00047-12
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1577637429

Abstract

Early targeted antimicrobial therapy helps saving costs and prevents the spread of antimicrobial resistances including in Escherichia coli, the most frequent gram-negative bacterium causing sepsis. Therefore, rapid susceptibility testing represents the major prerequisite for knowledge-based successful antimicrobial treatment. To accelerate testing for antibiotic susceptibility, we have developed a new mass spectrometry-based assay for antibiotic susceptibility testing (MAAST). For proof of principle we present an ampicillin susceptibility test for E.coli with a TAT of 90 minutes upon growth detection.