| Online-Ressource |
Verfasst von: | Sevko, Alexandra [VerfasserIn]  |
| Kremer, Veronika [VerfasserIn]  |
| Umansky, Ludmila [VerfasserIn]  |
| Umansky, Viktor [VerfasserIn]  |
Titel: | Application of paclitaxel in low non-cytotoxic doses supports vaccination with melanoma antigens in normal mice |
Verf.angabe: | Alexandra Sevko, Veronika Kremer, Christine Falk, Ludmila Umansky, Michael R. Shurin, Galina V. Shurin and Viktor Umansky |
E-Jahr: | 2012 |
Jahr: | 27 Mar 2012 |
Umfang: | 7 S. |
Fussnoten: | Published online: 27 Mar 2012 ; Gesehen am 16.07.2018 |
Titel Quelle: | Enthalten in: Journal of immunotoxicology |
Ort Quelle: | London [u.a.] : Taylor and Francis Group, 2004 |
Jahr Quelle: | 2012 |
Band/Heft Quelle: | 9(2012), 3, Seite 275-281 |
ISSN Quelle: | 1547-6901 |
Abstract: | Chemotherapeutic agents such as paclitaxel applied in ultra-low, non-cytotoxic doses were previously shown to stimulate dendritic cell activity and anti-tumor immune responses upon vaccination in mouse transplantable tumor models. However, the mechanisms of these alterations-termed chemoimmunomodulation or chemomodulation-are still not clear. This study investigated the effect of paclitaxel applied in ultra-low, non-cytotoxic doses on the efficiency of immunization of healthy C57BL/6 mice with the peptide derived from tyrosinase related protein (TRP)-2 as a model melanoma antigen. Using an IFNγ ELISPOT assay, it was found that administration of 1 mg paclitaxel/kg in combination with the peptide vaccination strongly increased the frequencies of TRP-2 specific spleen T-cells as compared to levels due to the vaccination alone. This was associated with a significant decrease in the levels of regulatory T-cells (Treg) and immature myeloid cells (known as a counterpart of myeloid derived suppressor cells [MDSC] in healthy mice). Such impairments of potential immunosuppressive cells were found to correlate with a strong increase in the amount of effector CD8+ and CD4+ T-cells in the bone marrow and spleen. Furthermore, in paclitaxel-treated mice, a significant augmentation of natural killer (NK) cell numbers in the bone marrow and their ability to produce IFNγ were observed. In addition, the level of NK-T-cells in the lymph nodes was also increased. It is suggested that paclitaxel applied in ultra-low, non-cytotoxic doses may potentially enhance the efficacy of anti-tumor vaccinations by neutralizing immunosuppressive Treg and MDSC populations in tumor-bearing hosts. |
DOI: | doi:10.3109/1547691X.2012.655343 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: http://dx.doi.org/10.3109/1547691X.2012.655343 |
| kostenfrei: Volltext: https://doi.org/10.3109/1547691X.2012.655343 |
| DOI: https://doi.org/10.3109/1547691X.2012.655343 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | chemoimmunomodulation |
| immature myeloid cells |
| myeloid-derived suppressor cells |
| Non-cytotoxic chemotherapy |
| paclitaxel |
| peptide immunization |
| regulatory T-cells |
K10plus-PPN: | 1577643259 |
Verknüpfungen: | → Zeitschrift |
Application of paclitaxel in low non-cytotoxic doses supports vaccination with melanoma antigens in normal mice / Sevko, Alexandra [VerfasserIn]; 27 Mar 2012 (Online-Ressource)