Bacterial quorum sensing molecule induces chemotaxis of human neutrophils via induction of p38 and leukocyte specific protein 1 (LSP1)

• Verfasst von: Kahle, Nadine [VerfasserIn]
• Verfasst von: Hänsch, Gertrud Maria [VerfasserIn]
• Titel: Bacterial quorum sensing molecule induces chemotaxis of human neutrophils via induction of p38 and leukocyte specific protein 1 (LSP1)
• Verf.angabe: N.A. Kahle, G. Brenner-Weiss, J. Overhage, U. Obst, G.M. Hänsch
• Jahr: 2013
• Jahr des Originals: 2012
• Umfang: 7 S.
• Fussnoten: Gesehen am 22.07.2018 ; Available online: 14 February 2012
• Titel Quelle: Enthalten in: Immunobiology
• Ort Quelle: München : Elsevier, 1979
• Jahr Quelle: 2013
• Band/Heft Quelle: 218(2013), 2, Seite 145-151
• ISSN Quelle: 1878-3279
• DOI: doi:10.1016/j.imbio.2012.02.004
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Acyl homoserine lactones (AHLs)
• Sach-SW: Chemotaxis
• Sach-SW: Human neutrophils
• Sach-SW: LSP1
• Sach-SW: p38 Map Kinase
• Sach-SW: Quorum sensing molecules
• K10plus-PPN: 1577816757

Abstract

When bacteria colonize surfaces, they socialize and form biofilms. This process is well regulated and relies on the communication among the bacteria via so-called “quorum sensing molecules”. Among those, N-(3-oxododecanoyl)-l-homoserine lactone (AHL-12), generated by Pseudomonas aeruginosa and other Gram-negative bacteria, activates not only bacteria but also interacts with mammalian cells. Among others, it activates phagocytic cells and - as we had shown previously - it is chemotactic for human polymorphonuclear neutrophils (PMN) in vitro. In the present study, we analyzed the signalling pathway of AHL-12 in PMN. We focused on the mitogen activated protein (MAP) kinase p38, because SB203580, an inhibitor of p38, prevented the AHL-12 induced chemotaxis. We found that in response to AHL-12, p38 was phosphorylated within minutes, as was its downstream target, the MAPKAP-Kinase-2 (MK2). In PMN, the major substrate of MK2 is the leukocyte specific protein 1 (LSP1), which binds to F-actin and participates directly in actin polymerization and cell migration. In response to AHL-12, LSP1 was phosphorylated and co-localized with F-actin in polarized PMN, suggesting that AHL-12-induced migration depended on p38 and LSP1 activation.