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Verfasst von:Sommaggio, Roberta [VerfasserIn]   i
 Cohnen, André [VerfasserIn]   i
 Watzl, Carsten [VerfasserIn]   i
Titel:Multiple receptors trigger human NK cell-mediated cytotoxicity against porcine chondrocytes
Verf.angabe:Roberta Sommaggio, André Cohnen, Carsten Watzl, and Cristina Costa
E-Jahr:2012
Jahr:February 17, 2012
Umfang:9 S.
Teil:volume:188
 year:2012
 number:5
 pages:2075-2083
 extent:9
Fussnoten:Gesehen am 24.07.2018
Titel Quelle:Enthalten in: The journal of immunology
Ort Quelle:Bethesda, Md. : Soc., 1916
Jahr Quelle:2012
Band/Heft Quelle:188(2012), 5, Seite 2075-2083
ISSN Quelle:1550-6606
Abstract:Xenotransplantation of genetically engineered porcine chondrocytes may provide a therapeutic solution for the repair of cartilage defects of various types. However, the mechanisms underlying the humoral and cellular responses that lead to rejection of xenogeneic cartilage are not well understood. In this study, we investigated the interaction between human NK cells and isolated porcine costal chondrocytes (PCC). Our data show that freshly isolated NK cells adhere weakly to PCC. Consequently, PCC were highly resistant to cytolysis mediated by freshly isolated NK cells. However, the presence of human natural Abs in the coculture was often sufficient to trigger cytotoxicity against PCC. Furthermore, IL-2 stimulation of NK cells or activation of PCC with the proinflammatory cytokines TNF-α or IL-1α resulted in increased adhesion, which was paralleled by increased NK cell-mediated lysis of PCC. NK cell adhesion to PCC could be blocked by Abs against human LFA-1 and porcine VCAM-1. NKG2D and NKp44 were involved in triggering cytotoxicity against PCC, which expressed ligands for these activating NK cell receptors. Our data further suggest that NKp30 and NKp46 may contribute to the activation of NK cells by PCC under certain conditions. Finally, comparative studies confirmed that PCC are more resistant than porcine aortic endothelial cells to human NK cell-mediated lysis. Thus, the data demonstrate that human NK cells can kill pig chondrocytes and may therefore contribute to rejection of xenogeneic cartilage. In addition, we identify potential targets for intervention to prevent the NK cell response against pig xenografts.
DOI:doi:10.4049/jimmunol.1100433
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Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.4049/jimmunol.1100433
 Kostenfrei: Volltext: http://www.jimmunol.org/content/188/5/2075
 DOI: https://doi.org/10.4049/jimmunol.1100433
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1577869613
Verknüpfungen:→ Zeitschrift

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