Status: Bibliographieeintrag
Standort: ---
Exemplare:
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| Online-Ressource |
Verfasst von: | Song, Chenlin [VerfasserIn]  |
| Hotz-Wagenblatt, Agnes [VerfasserIn]  |
| Voit, Renate [VerfasserIn]  |
| Grummt, Ingrid [VerfasserIn]  |
Titel: | SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability |
Verf.angabe: | Chenlin Song, Agnes Hotz-Wagenblatt, Renate Voit, and Ingrid Grummt |
E-Jahr: | 2017 |
Jahr: | August 8, 2017 |
Umfang: | 12 S. |
Fussnoten: | Published in Advance August 8, 2017 ; Gesehen am 24.07.2018 |
Titel Quelle: | Enthalten in: Genes & development |
Ort Quelle: | Stanford, Calif. : HighWire Press, 1987 |
Jahr Quelle: | 2017 |
Band/Heft Quelle: | 31(2017), 13, Seite 1370-1381 |
ISSN Quelle: | 1549-5477 |
Abstract: | R loops are three-stranded nucleic acid structures consisting of an RNA:DNA heteroduplex and a “looped-out” nontemplate strand. As aberrant formation and persistence of R loops block transcription elongation and cause DNA damage, mechanisms that resolve R loops are essential for genome stability. Here we show that the DEAD (Asp-Glu-Ala-Asp)-box RNA helicase DDX21 efficiently unwinds R loops and that depletion of DDX21 leads to accumulation of cellular R loops and DNA damage. Significantly, the activity of DDX21 is regulated by acetylation. Acetylation by CBP inhibits DDX21 activity, while deacetylation by SIRT7 augments helicase activity and overcomes R-loop-mediated stalling of RNA polymerases. Knockdown of SIRT7 leads to the same phenotype as depletion of DDX21 (i.e., increased formation of R loops and DNA double-strand breaks), indicating that SIRT7 and DDX21 cooperate to prevent R-loop accumulation, thus safeguarding genome integrity. Moreover, DDX21 resolves estrogen-induced R loops on estrogen-responsive genes in breast cancer cells, which prevents the blocking of transcription elongation on these genes. |
DOI: | doi:10.1101/gad.300624.117 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1101/gad.300624.117 |
| kostenfrei: Volltext: http://genesdev.cshlp.org/content/31/13/1370 |
| DOI: https://doi.org/10.1101/gad.300624.117 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | acetylation |
| DDX21 helicase |
| DNA damage |
| R loops |
| SIRT7 |
| transcription |
K10plus-PPN: | 1577879414 |
Verknüpfungen: | → Zeitschrift |
SIRT7 and the DEAD-box helicase DDX21 cooperate to resolve genomic R loops and safeguard genome stability / Song, Chenlin [VerfasserIn]; August 8, 2017 (Online-Ressource)
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