7-Aminoactinomycin D for apoptosis staining in flow cytometry

• Verfasst von: Zembruski, Nadine Cécile Luise [VerfasserIn]
• Verfasst von: Stache, Vanessa [VerfasserIn]
• Verfasst von: Haefeli, Walter E. [VerfasserIn]
• Verfasst von: Weiß, Johanna [VerfasserIn]
• Titel: 7-Aminoactinomycin D for apoptosis staining in flow cytometry
• Verf.angabe: Nadine C.L. Zembruski, Vanessa Stache, Walter E. Haefeli, Johanna Weiss
• E-Jahr: 2012
• Jahr: 14 July 2012
• Umfang: 3 S.
• Fussnoten: Gesehen am 21.07.2020
• Titel Quelle: Enthalten in: Analytical biochemistry
• Ort Quelle: San Diego, Calif. : Elsevier, 1960
• Jahr Quelle: 2012
• Band/Heft Quelle: 429(2012), 1, Seite 79-81
• ISSN Quelle: 1096-0309
• DOI: doi:10.1016/j.ab.2012.07.005
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: 7-AAD
• Sach-SW: Apoptosis
• Sach-SW: Flow cytometry
• K10plus-PPN: 1577902777

Abstract

7-Aminoactinomycin D (7-AAD) is a DNA dye that distinguishes viable, apoptotic, and late apoptotic/dead cells in flow cytometry. Several staining protocols using 7-AAD have been described, but data on the influence of the 7-AAD concentration on the readout are not available. Therefore, we compared the results obtained by staining with 1, 5, 10, and 20μg/ml 7-AAD for 20min with the PE-Annexin V Apoptosis Detection Kit and Cell Death Detection ELISAPLUS in lymphocytes and CEM human leukemia cells. The results showed that 7-AAD staining with 5, 10, and 20μg/ml, but not with 1μg/ml, is suitable for quantification of apoptosis in flow cytometry.