Status: Bibliographieeintrag
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| Verfasst von: | Vindry, Caroline [VerfasserIn]  |
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| | Stoecklin, Georg [VerfasserIn] |
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| Titel: | Dual RNA processing roles of Pat1b via cytoplasmic Lsm1-7 and nuclear Lsm2-8 complexes |
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| Verf.angabe: | Caroline Vindry, Aline Marnef, Helen Broomhead, Laure Twyffels, Sevim Ozgur, Georg Stoecklin, Miriam Llorian, Christopher W. Smith, Juan Mata, Dominique Weil, Nancy Standart |
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| E-Jahr: | 2017 |
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| Jahr: | August 1, 2017 |
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| Umfang: | 14 S. |
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| Fussnoten: | Gesehen am 25.07.2018 |
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| Titel Quelle: | Enthalten in: Cell reports |
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| Ort Quelle: | Maryland Heights, MO : Cell Press, 2012 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 20(2017), 5, Seite 1187-1200 |
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| ISSN Quelle: | 2211-1247 |
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| Abstract: | Pat1 RNA-binding proteins, enriched in processing bodies (P bodies), are key players in cytoplasmic 5′ to 3′ mRNA decay, activating decapping of mRNA in complex with the Lsm1-7 heptamer. Using co-immunoprecipitation and immunofluorescence approaches coupled with RNAi, we provide evidence for a nuclear complex of Pat1b with the Lsm2-8 heptamer, which binds to the spliceosomal U6 small nuclear RNA (snRNA). Furthermore, we establish the set of interactions connecting Pat1b/Lsm2-8/U6 snRNA/SART3 and additional U4/U6.U5 tri-small nuclear ribonucleoprotein particle (tri-snRNP) components in Cajal bodies, the site of snRNP biogenesis. RNA sequencing following Pat1b depletion revealed the preferential upregulation of mRNAs normally found in P bodies and enriched in 3′ UTR AU-rich elements. Changes in >180 alternative splicing events were also observed, characterized by skipping of regulated exons with weak donor sites. Our data demonstrate the dual role of a decapping enhancer in pre-mRNA processing as well as in mRNA decay via distinct nuclear and cytoplasmic Lsm complexes. |
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| DOI: | doi:10.1016/j.celrep.2017.06.091 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Volltext ; Verlag: http://dx.doi.org/10.1016/j.celrep.2017.06.091 |
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| | Kostenfrei: Volltext: http://www.sciencedirect.com/science/article/pii/S2211124717309415 |
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| | DOI: https://doi.org/10.1016/j.celrep.2017.06.091 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | alternative splicing |
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| | Cajal bodies |
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| | Lsm |
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| | mRNA decay |
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| | P bodies |
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| | Pat1b |
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| | PATL1 |
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| | Prp31 |
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| | SART3 |
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| | tri-snRNP |
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| K10plus-PPN: | 1577942639 |
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| Verknüpfungen: | → Zeitschrift |
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Dual RNA processing roles of Pat1b via cytoplasmic Lsm1-7 and nuclear Lsm2-8 complexes / Vindry, Caroline [VerfasserIn]; August 1, 2017 (Online-Ressource)
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