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Verfasst von:Nawrotzki, Ralph [VerfasserIn]   i
 Islinger, Markus [VerfasserIn]   i
 Vogel, Ingeborg [VerfasserIn]   i
 Völkl, Alfred [VerfasserIn]   i
 Kirsch, Joachim [VerfasserIn]   i
Titel:Expression and subcellular distribution of gephyrin in non-neuronal tissues and cells
Verf.angabe:Ralph Nawrotzki, Markus Islinger, Ingeborg Vogel, Alfred Völkl, Joachim Kirsch
E-Jahr:2012
Jahr:22 January 2012
Umfang:12 S.
Fussnoten:Published online: 22 January 2012 ; Gesehen am 26.07.2018
Titel Quelle:Enthalten in: Histochemistry and cell biology
Ort Quelle:Berlin : Springer, 1958
Jahr Quelle:2012
Band/Heft Quelle:137(2012), 4, Seite 471-482
ISSN Quelle:1432-119X
Abstract:Gephyrin is a scaffolding protein required for the accumulation of inhibitory neurotransmitter receptors at neuronal postsynaptic membranes. In non-neuronal tissues, gephyrin is indispensible for the biosynthesis of molybdenum cofactor, the prosthetic group of oxidoreductases including sulfite oxidase and xanthine oxidase. However, the molecular and cellular basis of gephyrin’s non-neuronal function is poorly understood; in particular, the roles of its splice variants remain enigmatic. Here, we used cDNA screening as well as Northern and immunoblot analyses to show that mammalian liver contains only a limited number of gephyrin splice variants, with the C3-containing variant being the predominant isoform. Using new and established anti-gephyrin antibodies in immunofluorescence and subcellular fractionation studies, we report that gephyrin localizes to the cytoplasm of both tissue hepatocytes and cultured immortalized cells. These findings were corroborated by RNA interference studies in which the cytosolic distribution was found to be abolished. Finally, by blue-native PAGE we show that cytoplasmic gephyrin is part of a ~600 kDa protein complex of yet unknown composition. Our data suggest that the expression pattern of non-neuronal gephyrin is simpler than indicated by previous evidence. In addition, gephyrin’s presence in a cytosolic 600 kDa protein complex suggests that its metabolic and/or other non-neuronal functions are exerted in the cytoplasm and are not confined to a particular subcellular compartment.
DOI:doi:10.1007/s00418-012-0914-7
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Volltext: http://dx.doi.org/10.1007/s00418-012-0914-7
 Volltext: https://link.springer.com/article/10.1007/s00418-012-0914-7
 DOI: https://doi.org/10.1007/s00418-012-0914-7
Datenträger:Online-Ressource
Sprache:eng
K10plus-PPN:1577986067
Verknüpfungen:→ Zeitschrift

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