Buffer-dependent regulation of aquaporin-1 expression and function in human peritoneal mesothelial cells

• Verfasst von: Zhai, Yihui [VerfasserIn]
• Verfasst von: Bloch, Jacek [VerfasserIn]
• Verfasst von: Hömme, Meike [VerfasserIn]
• Verfasst von: Schaefer, Julia [VerfasserIn]
• Verfasst von: Hackert, Thilo [VerfasserIn]
• Verfasst von: Philippin, Bärbel [VerfasserIn]
• Verfasst von: Schwenger, Vedat [VerfasserIn]
• Verfasst von: Schaefer, Franz [VerfasserIn]
• Verfasst von: Schmitt, Claus P. [VerfasserIn]
• Titel: Buffer-dependent regulation of aquaporin-1 expression and function in human peritoneal mesothelial cells
• Verf.angabe: Yihui Zhai, Jacek Bloch, Meike Hömme, Julia Schaefer, Thilo Hackert, Bärbel Philippin, Vedat Schwenger, Franz Schaefer, Claus P. Schmitt
• E-Jahr: 2012
• Jahr: 1 March 2012
• Umfang: 13 S.
• Teil: volume:27
• Teil: year:2012
• Teil: number:7
• Teil: pages:1165-1177
• Teil: extent:13
• Fussnoten: Gesehen am 27.07.2018
• Titel Quelle: Enthalten in: Pediatric nephrology
• Ort Quelle: Berlin : Springer, 1987
• Jahr Quelle: 2012
• Band/Heft Quelle: 27(2012), 7, Seite 1165-1177
• ISSN Quelle: 1432-198X
• DOI: doi:10.1007/s00467-012-2120-1
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1578029732

Abstract

BackgroundBiocompatible peritoneal dialysis fluids (PDF) are buffered with lactate and/or bicarbonate. We hypothesized that the reduced toxicity of the biocompatible solutions might unmask specific effects of the buffer type on mesothelial cell functions.MethodsHuman peritoneal mesothelial cells (HPMC) were incubated with bicarbonate (B-)PDF or lactate-buffered (L-)PDF followed by messenger RNA (mRNA) and protein analysis. Gene silencing was achieved using small interfering RNA (siRNA), functional studies using Transwell culture systems, and monolayer wound-healing assays.ResultsIncubation with B-PDF increased HPMC migration in the Transwell and monolayer wound-healing assay to 245 ± 99 and 137 ± 11% compared with L-PDF. Gene silencing showed this effect to be entirely dependent on the expression of aquaporin-1 (AQP-1) and independent of AQP-3. Exposure of HPMC to B-PDF increased AQP-1 mRNA and protein abundance to 209 ± 80 and 197 ± 60% of medium control; the effect was pH dependent. L-PDF reduced AQP-1 mRNA. Addition of bicarbonate to L-PDF increased AQP-1 abundance by threefold; mRNA half-life remained unchanged. Immunocytochemistry confirmed opposite changes of AQP-1 cell-membrane abundance with B-PDF and L-PDF.ConclusionsPeritoneal mesothelial AQP-1 abundance and migration capacity is regulated by pH and buffer agents used in PD solutions. In vivo studies are required to delineate the impact with respect to long-term peritoneal membrane integrity and function.