Capsaicin and piperine can overcome multidrug resistance in cancer cells to doxorubicin

• Verfasst von: Li, Hanmei [VerfasserIn]
• Verfasst von: Krstin, Sonja [VerfasserIn]
• Verfasst von: Wang, Shihui [VerfasserIn]
• Verfasst von: Wink, Michael [VerfasserIn]
• Titel: Capsaicin and piperine can overcome multidrug resistance in cancer cells to doxorubicin
• Verf.angabe: Hanmei Li, Sonja Krstin, Shihui Wang and Michael Wink
• E-Jahr: 2018
• Jahr: 2 March 2018
• Umfang: 11 S.
• Fussnoten: Gesehen am 30.07.2018
• Titel Quelle: Enthalten in: Molecules
• Ort Quelle: Basel : MDPI, 1996
• Jahr Quelle: 2018
• Band/Heft Quelle: 23(2018,3) Artikel-Nummer 557, 11 Seiten
• ISSN Quelle: 1420-3049
• DOI: doi:10.3390/molecules23030557
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Caco-2
• Sach-SW: capsaicin
• Sach-SW: CCRF-CEM
• Sach-SW: CEM/ADR 5000
• Sach-SW: HCT 116
• Sach-SW: multidrug resistance
• Sach-SW: piperine
• K10plus-PPN: 1578047412
• K10plus-PPN:
  Universitätsbibliothek
• Lokale URL UB: Zum Volltext

Abstract

Background: Multidrug resistance (MDR) can develop in cancer cells after treatment with anticancer drugs, mainly due to the overexpression of the ATP-binding cassette (ABC) transporters. We analyzed the ability of two pungent-tasting alkaloids—capsaicin and piperine from Capsicum frutescens and Piper nigrum, respectively—to reverse multidrug resistance in the cancer cell lines Caco-2 and CEM/ADR 5000, which overexpress P-glycoprotein (P-gp) and other ABC transporters. Methods: The MTT assay was first used to determine the cytotoxicity of doxorubicin, the alkaloids, and digitonin alone, and then their combinations. Furthermore, rhodamine (Rho) 123 and calcein-AM were used to detect the effects of alkaloids on the activity of P-gp. Results: Capsaicin and piperine synergistically enhanced the cytotoxicity of doxorubicin in Caco-2 and CEM/ADR 5000 cells. Furthermore, capsaicin and piperine increased the intracellular accumulation of the fluorescent P-glycoprotein (P-gp) substrates rhodamine and calcein and inhibited their efflux from the MDR cell lines. Conclusion: Our study has demonstrated that capsaicin and piperine are P-gp substrates and have potential chemosensitizing activity, which might be interesting for the development of novel modulators of multidrug resistance.