Navigation überspringen
Universitätsbibliothek Heidelberg
Status: Bibliographieeintrag

Verfügbarkeit
Standort: ---
Exemplare: ---
heiBIB
 Online-Ressource
Verfasst von:Zhan, Tianzuo [VerfasserIn]   i
 Poppelreuther, Margarete [VerfasserIn]   i
 Ehehalt, Robert [VerfasserIn]   i
 Füllekrug, Joachim [VerfasserIn]   i
Titel:Overexpressed FATP1, ACSVL4/FATP4 and ACSL1 increase the cellular fatty acid uptake of 3T3-L1 adipocytes but are localized on intracellular membranes
Verf.angabe:Tianzuo Zhan, Margarete Poppelreuther, Robert Ehehalt, Joachim Füllekrug
E-Jahr:2012
Jahr:September 14, 2012
Umfang:12 S.
Fussnoten:Gesehen am 30.07.2018
Titel Quelle:Enthalten in: PLOS ONE
Ort Quelle:San Francisco, California, US : PLOS, 2006
Jahr Quelle:2012
Band/Heft Quelle:7(2012,9) Artikel-Nummer e45087, 12 Seiten
ISSN Quelle:1932-6203
Abstract:Long chain acyl-CoA synthetases are essential enzymes of lipid metabolism, and have also been implicated in the cellular uptake of fatty acids. It is controversial if some or all of these enzymes have an additional function as fatty acid transporters at the plasma membrane. The most abundant acyl-CoA synthetases in adipocytes are FATP1, ACSVL4/FATP4 and ACSL1. Previous studies have suggested that they increase fatty acid uptake by direct transport across the plasma membrane. Here, we used a gain-of-function approach and established FATP1, ACSVL4/FATP4 and ACSL1 stably expressing 3T3-L1 adipocytes by retroviral transduction. All overexpressing cell lines showed increased acyl-CoA synthetase activity and fatty acid uptake. FATP1 and ACSVL4/FATP4 localized to the endoplasmic reticulum by confocal microscopy and subcellular fractionation whereas ACSL1 was found on mitochondria. Insulin increased fatty acid uptake but without changing the localization of FATP1 or ACSVL4/FATP4. We conclude that overexpressed acyl-CoA synthetases are able to facilitate fatty acid uptake in 3T3-L1 adipocytes. The intracellular localization of FATP1, ACSVL4/FATP4 and ACSL1 indicates that this is an indirect effect. We suggest that metabolic trapping is the mechanism behind the influence of acyl-CoA synthetases on cellular fatty acid uptake.
DOI:doi:10.1371/journal.pone.0045087
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

kostenfrei: Volltext: http://dx.doi.org/10.1371/journal.pone.0045087
 kostenfrei: Volltext: http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0045087
 DOI: https://doi.org/10.1371/journal.pone.0045087
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Adipocytes
 Cell membranes
 Fatty acids
 Fibroblasts
 Insulin
 Intracellular membranes
 Membrane proteins
 Mitochondria
K10plus-PPN:1578064287
Verknüpfungen:→ Zeitschrift

Permanenter Link auf diesen Titel (bookmarkfähig):  https://katalog.ub.uni-heidelberg.de/titel/68290173   QR-Code
zum Seitenanfang