| |  Online-Ressource |
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| Verfasst von: | Arif, Rawa [VerfasserIn]  |
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| | Zaradzki, Marcin [VerfasserIn] |
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| | Remes, Anca [VerfasserIn] |
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| | Seppelt, Philipp [VerfasserIn] |
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| | Kunze, Reiner [VerfasserIn] |
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| | Schröder, Hannes [VerfasserIn] |
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| | Schwill, Simon [VerfasserIn] |
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| | Karck, Matthias [VerfasserIn] |
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| | Müller, Oliver J. [VerfasserIn] |
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| | Hecker, Markus [VerfasserIn] |
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| | Wagner, Andreas H. [VerfasserIn] |
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| | Kallenbach, Klaus [VerfasserIn] |
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| Titel: | AP-1 oligodeoxynucleotides reduce aortic elastolysis in a murine model of marfan syndrome |
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| Verf.angabe: | Rawa Arif, Marcin Zaradzki, Anca Remes, Philipp Seppelt, Reiner Kunze, Hannes Schröder, Simon Schwill, Stephan M. Ensminger, Peter N. Robinson, Matthias Karck, Oliver J. Müller, Markus Hecker, Andreas H. Wagner, Klaus Kallenbach |
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| E-Jahr: | 2017 |
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| Jahr: | 20 September 2017 |
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| Umfang: | 11 S. |
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| Fussnoten: | Available online 20 September 2017 ; Gesehen am 31.07.2018 |
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| Titel Quelle: | Enthalten in: Molecular Therapy / Nucleic Acids |
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| Ort Quelle: | New York, NY : Nature Publ. Group, 2012 |
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| Jahr Quelle: | 2017 |
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| Band/Heft Quelle: | 9(2017), Seite 69-79 |
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| ISSN Quelle: | 2162-2531 |
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| Abstract: | Marfan syndrome is characterized by high expression of matrix metalloproteinases (MMPs) in aortic smooth muscle cells (AoSMCs) associated with medial elastolysis and aortic root aneurysm. We aimed to reduce aortic elastolysis through decrease of MMP expression with decoy oligodeoxynucleotides (dODNs) neutralizing the transcription factor activating factor-1 (AP-1). AP-1 abundance in nuclear extracts as well as MMP-2 and MMP-9 expression were significantly increased in isolated mAoSMC of mgR/mgR Marfan mice compared to wild-type cells. Exposure to AP-1 neutralizing dODNs resulted in a significant reduction of basal and interleukin-1β-stimulated MMP expression and activity in mAoSMCs. Moreover, increased migration and formation of superoxide radical anions was substantially decreased in mAoSMCs by AP-1 dODN treatment. Aortic grafts from donor Marfan mice were treated with AP-1- dODN ex vivo and implanted as infrarenal aortic interposition grafts in mgR/mgR mice. Pretreatment of aortic grafts with AP-1 dODN led to reduced elastolysis, macrophage infiltration, and MMP activity. Permeability of the endothelial monolayer was increased for dODN in mgR/mgR aortae with observed loss of tight junction proteins ZO-1 and occludin, enabling dODN to reach the tunica media. Targeting AP-1 activity offers a new potential strategy to treat the vascular phenotype associated with Marfan syndrome. |
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| DOI: | doi:10.1016/j.omtn.2017.08.014 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
kostenfrei: Volltext: http://dx.doi.org/10.1016/j.omtn.2017.08.014 |
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| | DOI: https://doi.org/10.1016/j.omtn.2017.08.014 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | aneurysm |
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| | aorta |
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| | AP-1 |
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| | Marfan syndrome |
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| | matrix metalloproteinases |
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| K10plus-PPN: | 1578108500 |
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| Verknüpfungen: | → Zeitschrift |
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AP-1 oligodeoxynucleotides reduce aortic elastolysis in a murine model of marfan syndrome / Arif, Rawa [VerfasserIn]; 20 September 2017 (Online-Ressource)
