Evaluation of Alere i RSV for rapid detection of respiratory syncytial virus in children hospitalized with acute respiratory tract infection

• Verfasst von: Peters, Rebecca [VerfasserIn]
• Verfasst von: Schnee, Sarah Valerie [VerfasserIn]
• Verfasst von: Tabatabai, Julia [VerfasserIn]
• Verfasst von: Schnitzler, Paul [VerfasserIn]
• Verfasst von: Pfeil, Johannes [VerfasserIn]
• Titel: Evaluation of Alere i RSV for rapid detection of respiratory syncytial virus in children hospitalized with acute respiratory tract infection
• Verf.angabe: Rebecca Marie Peters, Sarah Valerie Schnee, Julia Tabatabai, Paul Schnitzler, Johannes Pfeil
• E-Jahr: 2017
• Jahr: 11 January 2017
• Umfang: 5 S.
• Fussnoten: Accepted manuscript posted online11January 2017 ; Gesehen am 31.07.2018
• Titel Quelle: Enthalten in: Journal of clinical microbiology
• Ort Quelle: Washington, DC : Soc., 1975
• Jahr Quelle: 2017
• Band/Heft Quelle: 55(2017), 4, Seite 1032-1036
• ISSN Quelle: 1098-660X
• DOI: doi:10.1128/JCM.02433-16
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1578118891

Abstract

Alere™ i RSV is a novel rapid test which applies a nicking enzyme amplification reaction to detect respiratory syncytial virus in point-of-care settings. In this study, we evaluated the Alere™ i RSV assay by using frozen nasopharyngeal swab samples that were collected in viral transport medium from children hospitalized with acute respiratory tract infection during winter season 2015/2016. Alere™ i RSV assay test results were compared to Altona® RealStar RSV real-time reverse transcription PCR (RT-PCR). We found that the overall sensitivity and specificity of the Alere™ i RSV test assay was 100% (CI95 93% - 100%) and 97% (CI95 89% - 100%), respectively. Positive samples were identified within 5-7 minutes from sample collection. Overall, the Alere™ i RSV test assay performed well in comparison to the RT-PCR assay and has the potential to facilitate the detection of RSV in point-of-care settings.