| |  Online-Ressource |
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| Verfasst von: | Askoxylakis, Vasileios [VerfasserIn]  |
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| | Marr, Annabell [VerfasserIn] |
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| | Altmann, Annette [VerfasserIn] |
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| | Markert, Annette [VerfasserIn] |
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| | Mier, Walter [VerfasserIn] |
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| | Debus, Jürgen [VerfasserIn] |
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| | Huber, Peter E. [VerfasserIn] |
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| | Haberkorn, Uwe [VerfasserIn] |
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| Titel: | Peptide-based targeting of the platelet-derived growth factor receptor beta |
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| Verf.angabe: | Vasileios Askoxylakis, Annabell Marr, Annette Altmann, Annette Markert, Walter Mier, Jürgen Debus, Peter E. Huber, Uwe Haberkorn |
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| Jahr: | 2013 |
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| Jahr des Originals: | 2012 |
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| Umfang: | 10 S. |
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| Fussnoten: | Published online: 13 July 2012 ; Gesehen am 14.08.2018 |
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| Titel Quelle: | Enthalten in: Molecular imaging & biology |
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| Ort Quelle: | Cham : Springer Nature Switzerland, 2002 |
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| Jahr Quelle: | 2013 |
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| Band/Heft Quelle: | 15(2013), 2, Seite 212-221 |
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| ISSN Quelle: | 1860-2002 |
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| Abstract: | PURPOSE: The aim of this work is to identify new ligands targeting the platelet-derived growth factor receptor beta (PDGFRβ). PROCEDURES: Biopanning was carried out with a 12-amino-acid phage display library against the recombinant extracellular domain of PDGFRβ. The identified peptide PDGFR-P1 was chemically synthesized and labeled with (125)I or (131)I. In vitro studies were performed on the PDGFRβ-expressing cell lines BxPC3 and MCF7 and on PDGFRβ-transfected HEK cells in comparison to negative control wtHEK293 and CaIX-transfected HEK cells. Biodistribution experiments were performed in Balb/c nude mice, carrying subcutaneously BxPC3 tumors. RESULTS: In vitro studies demonstrated a higher binding to BxPC3, MCF7, and PDGFRβ-tr-HEK cells in comparison to negative control cell lines. Binding was inhibited up to 90% by the unlabeled PDGFR-P1 peptide. Organ distribution studies revealed a higher accumulation in BxPC3 tumors than in most organs. CONCLUSIONS: PDGFR-P1 is a promising candidate for targeting human PDGFRβ. |
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| DOI: | doi:10.1007/s11307-012-0578-7 |
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| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1007/s11307-012-0578-7 |
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| | DOI: https://doi.org/10.1007/s11307-012-0578-7 |
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| Datenträger: | Online-Ressource |
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| Sprache: | eng |
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| Sach-SW: | Amino Acid Sequence |
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| | Animals |
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| | Drug Delivery Systems |
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| | Drug Discovery |
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| | Female |
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| | HEK293 Cells |
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| | Humans |
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| | Immobilized Proteins |
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| | Ligands |
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| | MCF-7 Cells |
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| | Mice |
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| | Mice, Inbred BALB C |
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| | Mice, Nude |
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| | Molecular Sequence Data |
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| | Pancreatic Neoplasms |
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| | Peptide Library |
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| | Peptides |
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| | Receptor, Platelet-Derived Growth Factor beta |
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| | Transfection |
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| | Transplantation, Heterologous |
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| K10plus-PPN: | 1580012701 |
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| Verknüpfungen: | → Zeitschrift |
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Peptide-based targeting of the platelet-derived growth factor receptor beta / Askoxylakis, Vasileios [VerfasserIn]; 2013 (Online-Ressource)
