| Online-Ressource |
Verfasst von: | Askoxylakis, Vasileios [VerfasserIn]  |
| Marr, Annabell [VerfasserIn]  |
| Altmann, Annette [VerfasserIn]  |
| Markert, Annette [VerfasserIn]  |
| Mier, Walter [VerfasserIn]  |
| Debus, Jürgen [VerfasserIn]  |
| Huber, Peter E. [VerfasserIn]  |
| Haberkorn, Uwe [VerfasserIn]  |
Titel: | Peptide-based targeting of the platelet-derived growth factor receptor beta |
Verf.angabe: | Vasileios Askoxylakis, Annabell Marr, Annette Altmann, Annette Markert, Walter Mier, Jürgen Debus, Peter E. Huber, Uwe Haberkorn |
Jahr: | 2013 |
Jahr des Originals: | 2012 |
Umfang: | 10 S. |
Fussnoten: | Published online: 13 July 2012 ; Gesehen am 14.08.2018 |
Titel Quelle: | Enthalten in: Molecular imaging & biology |
Ort Quelle: | Cham : Springer Nature Switzerland, 2002 |
Jahr Quelle: | 2013 |
Band/Heft Quelle: | 15(2013), 2, Seite 212-221 |
ISSN Quelle: | 1860-2002 |
Abstract: | PURPOSE: The aim of this work is to identify new ligands targeting the platelet-derived growth factor receptor beta (PDGFRβ). PROCEDURES: Biopanning was carried out with a 12-amino-acid phage display library against the recombinant extracellular domain of PDGFRβ. The identified peptide PDGFR-P1 was chemically synthesized and labeled with (125)I or (131)I. In vitro studies were performed on the PDGFRβ-expressing cell lines BxPC3 and MCF7 and on PDGFRβ-transfected HEK cells in comparison to negative control wtHEK293 and CaIX-transfected HEK cells. Biodistribution experiments were performed in Balb/c nude mice, carrying subcutaneously BxPC3 tumors. RESULTS: In vitro studies demonstrated a higher binding to BxPC3, MCF7, and PDGFRβ-tr-HEK cells in comparison to negative control cell lines. Binding was inhibited up to 90% by the unlabeled PDGFR-P1 peptide. Organ distribution studies revealed a higher accumulation in BxPC3 tumors than in most organs. CONCLUSIONS: PDGFR-P1 is a promising candidate for targeting human PDGFRβ. |
DOI: | doi:10.1007/s11307-012-0578-7 |
URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Volltext: http://dx.doi.org/10.1007/s11307-012-0578-7 |
| DOI: https://doi.org/10.1007/s11307-012-0578-7 |
Datenträger: | Online-Ressource |
Sprache: | eng |
Sach-SW: | Amino Acid Sequence |
| Animals |
| Drug Delivery Systems |
| Drug Discovery |
| Female |
| HEK293 Cells |
| Humans |
| Immobilized Proteins |
| Ligands |
| MCF-7 Cells |
| Mice |
| Mice, Inbred BALB C |
| Mice, Nude |
| Molecular Sequence Data |
| Pancreatic Neoplasms |
| Peptide Library |
| Peptides |
| Receptor, Platelet-Derived Growth Factor beta |
| Transfection |
| Transplantation, Heterologous |
K10plus-PPN: | 1580012701 |
Verknüpfungen: | → Zeitschrift |
Peptide-based targeting of the platelet-derived growth factor receptor beta / Askoxylakis, Vasileios [VerfasserIn]; 2013 (Online-Ressource)