Super-resolved linear fluorescence localization microscopy using photostable fluorophores

• Verfasst von: Birk, Udo J. [VerfasserIn]
• Verfasst von: Szczurek, Aleksander [VerfasserIn]
• Verfasst von: Cremer, Christoph [VerfasserIn]
• Titel: Super-resolved linear fluorescence localization microscopy using photostable fluorophores
• Titelzusatz: a virtual microscopy study
• Verf.angabe: Udo Birk, Aleksander Szczurek, Christoph Cremer
• E-Jahr: 2017
• Jahr: 21 July 2017
• Umfang: 9 S.
• Fussnoten: Available online 21 July 2017 ; Gesehen am 14.08.2018
• Titel Quelle: Enthalten in: Optics communications
• Ort Quelle: Amsterdam, 1969
• Jahr Quelle: 2017
• Band/Heft Quelle: 404(2017), Seite 42-50
• ISSN Quelle: 1873-0310
• DOI: doi:10.1016/j.optcom.2017.06.078
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: Linear fluorescence microscopy
• Sach-SW: Photostable dyes
• Sach-SW: SMLM
• Sach-SW: Super-resolution fluorescence microscopy
• K10plus-PPN: 1580018246

Abstract

Current approaches to overcome the conventional limit of the resolution potential of light microscopy (of about 200 nm for visible light), often suffer from non-linear effects, which render the quantification of the image intensities in the reconstructions difficult, and also affect the quantification of the biological structure under investigation. As an attempt to face these difficulties, we discuss a particular method of localization microscopy which is based on photostable fluorescent dyes. The proposed method can potentially be implemented as a fast alternative for quantitative localization microscopy, circumventing the need for the acquisition of thousands of image frames and complex, highly dye-specific imaging buffers. Although the need for calibration remains in order to extract quantitative data (such as the number of emitters), multispectral approaches are largely facilitated due to the much less stringent requirements on imaging buffers. Furthermore, multispectral acquisitions can be readily obtained using commercial instrumentation such as e.g. the conventional confocal laser scanning microscope.