| |  Online-Ressource |
|---|
| Verfasst von: | Askoxylakis, Vasileios [VerfasserIn]  |
|---|
| | Ehemann, Volker [VerfasserIn] |
|---|
| | Rana, Shoaib [VerfasserIn] |
|---|
| | Krämer, Susanne [VerfasserIn] |
|---|
| | Rahbari, Nuh Nabi [VerfasserIn] |
|---|
| | Debus, Jürgen [VerfasserIn] |
|---|
| | Haberkorn, Uwe [VerfasserIn] |
|---|
| Titel: | Binding of the phage display derived peptide CaIX-P1 on human colorectal carcinoma cells correlates with the expression of carbonic anhydrase IX |
|---|
| Verf.angabe: | Vasileios Askoxylakis, Volker Ehemann, Shoaib Rana, Susanne Krämer, Nuh N. Rahbari, Jürgen Debus, Uwe Haberkorn |
|---|
| E-Jahr: | 2012 |
|---|
| Jahr: | 11 October 2012 |
|---|
| Umfang: | 19 S. |
|---|
| Teil: | volume:13 |
|---|
| | year:2012 |
|---|
| | number:10 |
|---|
| | pages:13030-13048 |
|---|
| | extent:19 |
|---|
| Fussnoten: | Gesehen am 15.08.2018 |
|---|
| Titel Quelle: | Enthalten in: International journal of molecular sciences |
|---|
| Ort Quelle: | Basel : Molecular Diversity Preservation International, 2000 |
|---|
| Jahr Quelle: | 2012 |
|---|
| Band/Heft Quelle: | 13(2012), 10, Seite 13030-13048 |
|---|
| ISSN Quelle: | 1422-0067 |
|---|
| | 1661-6596 |
|---|
| Abstract: | Phage display represents an attractive screening strategy for the identification of novel, specific binding ligands that could be used for tumor targeting. Recently, a new peptide (CaIX-P1) with affinity for human carbonic anhydrase IX (CAIX) was identified and evaluated. The aim of the present study is to characterize the properties of CaIX-P1 for targeting human colorectal carcinoma and investigate the correlation of peptide binding with the expression of carbonic anhydrase IX. Human colorectal carcinoma HCT116 and HT29 cells were investigated for CAIX expression using Western Blot analysis. Binding and competition studies of 125I-radiolabeled CaIX-P1 were performed on HCT116 cells in vitro. FACS analysis and fluorescence microscopy studies were carried out after cell incubation with fluorescein-labeled CaIX-P1 and rhodamine-labeled anti-human CAIX-mAb. Our studies revealed an enhanced in vitro expression of carbonic anhydrase IX in HCT116 and HT29 cells with increasing cell density. Binding of 125I-labeled-CaIX-P1 on HCT116 cells increased with increasing cell density and correlated to the CAIX expression. FACS analysis demonstrated a correlation of cell labeling between FITC-CaIX-P1 and rhodamine-labeled anti-CAIX-mAb in both HCT116 and HT29 cells. The results of our study indicate that the phage display identified peptide CaIX-P1 might be an attractive candidate for the development of a ligand targeting CAIX in colorectal cancer. |
|---|
| DOI: | doi:10.3390/ijms131013030 |
|---|
| URL: | Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.
Kostenfrei: Volltext: http://dx.doi.org/10.3390/ijms131013030 |
|---|
| | DOI: https://doi.org/10.3390/ijms131013030 |
|---|
| Datenträger: | Online-Ressource |
|---|
| Sprache: | eng |
|---|
| Sach-SW: | Antibodies, Monoclonal |
|---|
| | Antigens, Neoplasm |
|---|
| | Carbonic Anhydrase IX |
|---|
| | Carbonic Anhydrases |
|---|
| | Colorectal Neoplasms |
|---|
| | HCT116 Cells |
|---|
| | HT29 Cells |
|---|
| | Humans |
|---|
| | Kinetics |
|---|
| | Microscopy, Fluorescence |
|---|
| | Peptide Library |
|---|
| | Peptides |
|---|
| | Protein Binding |
|---|
| K10plus-PPN: | 1580062253 |
|---|
| Verknüpfungen: | → Zeitschrift |
|---|
Binding of the phage display derived peptide CaIX-P1 on human colorectal carcinoma cells correlates with the expression of carbonic anhydrase IX / Askoxylakis, Vasileios [VerfasserIn]; 11 October 2012 (Online-Ressource)
