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Verfasst von:Askoxylakis, Vasileios [VerfasserIn]   i
 Ehemann, Volker [VerfasserIn]   i
 Rana, Shoaib [VerfasserIn]   i
 Krämer, Susanne [VerfasserIn]   i
 Rahbari, Nuh Nabi [VerfasserIn]   i
 Debus, Jürgen [VerfasserIn]   i
 Haberkorn, Uwe [VerfasserIn]   i
Titel:Binding of the phage display derived peptide CaIX-P1 on human colorectal carcinoma cells correlates with the expression of carbonic anhydrase IX
Verf.angabe:Vasileios Askoxylakis, Volker Ehemann, Shoaib Rana, Susanne Krämer, Nuh N. Rahbari, Jürgen Debus, Uwe Haberkorn
E-Jahr:2012
Jahr:11 October 2012
Umfang:19 S.
Teil:volume:13
 year:2012
 number:10
 pages:13030-13048
 extent:19
Fussnoten:Gesehen am 15.08.2018
Titel Quelle:Enthalten in: International journal of molecular sciences
Ort Quelle:Basel : Molecular Diversity Preservation International, 2000
Jahr Quelle:2012
Band/Heft Quelle:13(2012), 10, Seite 13030-13048
ISSN Quelle:1422-0067
 1661-6596
Abstract:Phage display represents an attractive screening strategy for the identification of novel, specific binding ligands that could be used for tumor targeting. Recently, a new peptide (CaIX-P1) with affinity for human carbonic anhydrase IX (CAIX) was identified and evaluated. The aim of the present study is to characterize the properties of CaIX-P1 for targeting human colorectal carcinoma and investigate the correlation of peptide binding with the expression of carbonic anhydrase IX. Human colorectal carcinoma HCT116 and HT29 cells were investigated for CAIX expression using Western Blot analysis. Binding and competition studies of 125I-radiolabeled CaIX-P1 were performed on HCT116 cells in vitro. FACS analysis and fluorescence microscopy studies were carried out after cell incubation with fluorescein-labeled CaIX-P1 and rhodamine-labeled anti-human CAIX-mAb. Our studies revealed an enhanced in vitro expression of carbonic anhydrase IX in HCT116 and HT29 cells with increasing cell density. Binding of 125I-labeled-CaIX-P1 on HCT116 cells increased with increasing cell density and correlated to the CAIX expression. FACS analysis demonstrated a correlation of cell labeling between FITC-CaIX-P1 and rhodamine-labeled anti-CAIX-mAb in both HCT116 and HT29 cells. The results of our study indicate that the phage display identified peptide CaIX-P1 might be an attractive candidate for the development of a ligand targeting CAIX in colorectal cancer.
DOI:doi:10.3390/ijms131013030
URL:Bitte beachten Sie: Dies ist ein Bibliographieeintrag. Ein Volltextzugriff für Mitglieder der Universität besteht hier nur, falls für die entsprechende Zeitschrift/den entsprechenden Sammelband ein Abonnement besteht oder es sich um einen OpenAccess-Titel handelt.

Kostenfrei: Volltext: http://dx.doi.org/10.3390/ijms131013030
 DOI: https://doi.org/10.3390/ijms131013030
Datenträger:Online-Ressource
Sprache:eng
Sach-SW:Antibodies, Monoclonal
 Antigens, Neoplasm
 Carbonic Anhydrase IX
 Carbonic Anhydrases
 Colorectal Neoplasms
 HCT116 Cells
 HT29 Cells
 Humans
 Kinetics
 Microscopy, Fluorescence
 Peptide Library
 Peptides
 Protein Binding
K10plus-PPN:1580062253
Verknüpfungen:→ Zeitschrift

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