Time for genome editing

• Verfasst von: Singer, Mirko [VerfasserIn]
• Verfasst von: Frischknecht, Friedrich [VerfasserIn]
• Titel: Time for genome editing
• Titelzusatz: next-generation attenuated Malaria parasites
• Verf.angabe: Mirko Singer, Friedrich Frischknecht
• Jahr: 2017
• Jahr des Originals: 2016
• Umfang: 12 S.
• Fussnoten: Available online 25 October 2016 ; Gesehen am 15.08.2018
• Titel Quelle: Enthalten in: Trends in parasitology
• Ort Quelle: Amsterdam : Elsevier, 1985
• Jahr Quelle: 2017
• Band/Heft Quelle: 33(2017), 3, Seite 202-213
• ISSN Quelle: 1471-5007
• DOI: doi:10.1016/j.pt.2016.09.012
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: advanced genetics
• Sach-SW: CRISPR/CAS9
• Sach-SW: GAP
• Sach-SW: genome editing
• Sach-SW: ZFNs
• K10plus-PPN: 1580072992

Abstract

Immunization with malaria parasites that developmentally arrest in or immediately after the liver stage is the only way currently known to confer sterilizing immunity in both humans and rodent models. There are various ways to attenuate parasite development resulting in different timings of arrest, which has a significant impact on vaccination efficiency. To understand what most impacts vaccination efficiency, newly developed gain-of-function methods can now be used to generate a wide array of differently attenuated parasites. The combination of multiple attenuation approaches offers the potential to engineer efficiently attenuated Plasmodium parasites and learn about their fascinating biology at the same time. Here we discuss recent studies and the potential of targeted parasite manipulation using genome editing to develop live attenuated malaria vaccines.