Time-lapse imaging reveals differences in growth dynamics of embryos after in vitro maturation compared with conventional stimulation

• Verfasst von: Rösner, Sabine [VerfasserIn]
• Verfasst von: Dietrich, Jens Erik [VerfasserIn]
• Verfasst von: Weigert, Julia [VerfasserIn]
• Verfasst von: Strowitzki, Thomas [VerfasserIn]
• Titel: Time-lapse imaging reveals differences in growth dynamics of embryos after in vitro maturation compared with conventional stimulation
• Verf.angabe: Sabine Roesner, Jens Erik Dietrich, Julia Weigert, Markus Montag, Bettina Toth, and Thomas Strowitzki
• E-Jahr: 2017
• Jahr: 27 January 2017
• Umfang: 10 S.
• Fussnoten: Gesehen am 16.08.2018
• Titel Quelle: Enthalten in: Fertility and sterility
• Ort Quelle: Amsterdam [u.a.] : Elsevier Science, 1950
• Jahr Quelle: 2017
• Band/Heft Quelle: 107(2017), 3, Seite 606-612.e3
• ISSN Quelle: 1556-5653
• DOI: doi:10.1016/j.fertnstert.2016.12.026
• Datenträger: Online-Ressource
• Sprache: eng
• Sach-SW: clinical pregnancy rate
• Sach-SW: in vitro maturation
• Sach-SW: morphokinetic parameters
• Sach-SW: Time-lapse imaging
• K10plus-PPN: 1580099955

Abstract

Objective To study the impact of in vitro maturation (IVM) on embryonal development with the use of time-lapse imaging. Design Retrospective case-control study. Setting University hospital. Patient(s) In total, 294 embryos were cultured after intracytoplasmic sperm injection treatment of three groups: patients with polycystic ovary syndrome (PCOS) and IVM (n = 105; group 1 [G1]), patients after conventional stimulation without PCOS (n = 115; G2) and with PCOS (n = 74; G3). In total, 171 embryos were finally analyzed (57 G1, 65 G2, and 49 G3). Intervention(s) Data of 23 PCOS patients (30 IVM cycles) from January 2012 to July 2015 were matched according to age and number of oocytes to patients after conventional stimulation without PCOS (n = 30; 30 cycles) and with PCOS (n = 16; 19 cycles). Markers of embryo development were analyzed at different time points. Pregnancy rates (PRs) and live birth rates (LBRs) were recorded. Main Outcome Measure(s) Morphokinetic differences in embryo development after IVM compared with conventional stimulation with or without PCOS. Result(s) The rate of good-quality embryos was significantly lower in G1. Embryo development in G1 was significantly accelerated to the time of appearance of two pronuclei but slowed down by the time of reaching 6-cell stage and remained slower compared with embryos of G2 and G3. PRs as well as LBRs did not differ significantly among the study groups. Conclusion(s) Although growth dynamics of embryos from G1 differ from G2 and G3 and the rate of good-quality embryos was lower in IVM embryos, PRs and LBRs did not differ significantly.