Applying microdroplets as sensors for label-free detection of chemical reactions

• Verfasst von: Hofmann, Tobias [VerfasserIn]
• Verfasst von: Hänselmann, Siegfried [VerfasserIn]
• Verfasst von: Janiesch, Jan-Willi [VerfasserIn]
• Verfasst von: Rademacher, Anne [VerfasserIn]
• Verfasst von: Böhm, Christian H. J. [VerfasserIn]
• Titel: Applying microdroplets as sensors for label-free detection of chemical reactions
• Verf.angabe: Tobias W. Hofmann, Siegfried Hänselmann, Jan-Wilhelm Janiesch, Anne Rademacher, Christian H.J. Böhm
• E-Jahr: 2012
• Jahr: 17 Jan 2012
• Umfang: 7 S.
• Fussnoten: Gesehen am 16.08.2018
• Titel Quelle: Enthalten in: Lab on a chip
• Ort Quelle: Cambridge : RSC, 2001
• Jahr Quelle: 2012
• Band/Heft Quelle: 12(2012), 5, Seite 916-922
• ISSN Quelle: 1473-0189
• DOI: doi:10.1039/C2LC20971C
• Datenträger: Online-Ressource
• Sprache: eng
• K10plus-PPN: 1580112633

Abstract

Despite its tremendous high-throughput screening capabilities, widespread applications of droplet-based microfluidics are still limited by the poor availability of appropriate analytical assays. Here we report on a novel sensor method that exploits the osmosis-driven change in droplet size as a quantitative and label-free marker for reactions inside the droplets. We present an analysis of the underlying mechanism and apply the method for monitoring metabolic activity at a single-cell level.